Development of a Portable Rapid Detection Method for Porcine Epidemic Diarrhea Virus Using Reverse Transcription-Recombinase-Aided Amplification Technology
文献类型： 外文期刊
作者： Zhao, Yiran 1 ; Yi, Weijie 1 ; Yang, Qicheng 1 ; Li, Jiahao 2 ; Shan, Yanke 1 ; Liu, Fei 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Minist Educ, Joint Int Res Lab Anim Hlth & Food Safety ,Single, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Peoples R China
关键词： porcine epidemic diarrhea virus; isothermal amplification technology; reverse transcription-recombinase-aided amplification; point-of-care detection
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2025 年 15 卷 2 期
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea virus (PEDV) continues to spread globally, causing clinical symptoms in piglets, including watery diarrhea, vomiting, and dehydration. Its exceptionally high morbidity and mortality rate contributes significantly to the economic losses of the swine industry. The continuous genetic mutations of PEDV have compromised the effectiveness of classical strain vaccines. Early detection and accurate diagnosis are therefore crucial for controlling its further spread. Developing a detection method that is user-friendly, highly sensitive, and efficient is crucial for disease control. In this study, a point-of-care rapid detection method for PEDV was successfully established using reverse transcription-recombinase-aided amplification (RT-RAA) technology. This method enables results to be obtained within 20 min of amplification at a constant temperature of 42 degrees C. It demonstrates high sensitivity, with a detection limit as low as 1 copy/mu L, and shows strong specificity, with no cross-reactivity observed with seven other common swine pathogens. When applied to clinical samples, the results were 100% consistent with those obtained by RT-qPCR. This method is distinguished by its portable instrumentation and simple operation, making it particularly suitable for resource-constrained settings.
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