您好，欢迎访问海南省农业科学院 机构知识库！
海南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Plant miR167e-5p promotes 3T3-L1 adipocyte adipogenesis by targeting beta-catenin

文献类型： 外文期刊

作者： Chen, Ting 1 ; Ma, Fei 2 ; Peng, Yongjia 2 ; Sun, Ruiping 3 ; Xi, Qianyun 1 ; Sun, Jiajie 1 ; Zhang, Jin 2 ; Zhang, Yongliang 1 ; Li, Meng 2 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Engn Res Ctr Breeding Swine Ind, Coll Anim Sci,Guangdong Prov Key Lab Anim Nutr Co, 483 Wushan Rd, Guangzhou 510642, Peoples R China

2.Jiaxing Univ, Coll Biol Chem Sci & Engn, Jiaxing 314000, Peoples R China

3.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China

关键词： Exogenous; miRNA; Cross-kingdom; Adipogenesis

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.723； 五年影响因子：2.303 ）

ISSN： 1071-2690

年卷期： 2022 年 58 卷 6 期

页码：

收录情况： SCI

摘要： Adipogenesis is important in the development of fat deposition. Evidence showed that plant microRNAs (miRNAs) could be absorbed by the digestive tract and exert regulatory effects on animals' physiological processes. However, the regulation of plant miRNA on host lipogenesis remains unknown. This study explored the potential function of plant miRNA, miR167e-5p, in adipogenesis in vitro. The presentation of plant miR167e-5p improved lipid accumulation in 3T3-L1 cells. Bioinformatics prediction and luciferase reporter assay indicated that miR167e-5p targeted beta-catenin. MiR167e-5p could not only negatively affect the expression of beta-catenin but also showed a positive effect on several fat synthesis-related genes, peroxisome proliferator-activated receptor gamma (Ppary), CCAAT/enhancer-binding protein alpha (Cebp alpha), fatty acid-binding protein 4 (Ap2), lipolysis genes, adipose triglyceride lipase (Atgl), and hormone-sensitive lipase (Hsl) messenger RNA levels. Meanwhile, lipid accumulation and the expression of the beta-catenin and other five fat synthesis-related genes were recovered to their original pattern by adding the miR167e-5p inhibitor in 3T3-L1 cells. The immunoblot confirmed the same expression pattern in protein levels in beta-catenin, PPAR-gamma, FAS, and HSL. This research demonstrates that plant miR167e-5p can potentially affect adipogenesis through the regulation of beta-catenin, suggesting that plant miRNAs could be a new class of bioactive ingredients in adipogenesis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：海南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台