Comprehensive identification of cotton EPF/EPFL receptors and functional characterization of the GhEPFL1-1-GhER1 module in drought tolerance
文献类型： 外文期刊
作者： He, Shichang 1 ; Sun, Huijian 2 ; Chen, Qing 1 ; Yang, Yanlong 3 ; Zhou, Zixin 3 ; Chang, Saiwen 1 ; Lu, Shuaiqi 1 ; Liang, Zhencai 1 ; Yang, Julan 1 ; Fei, Xiao 1 ;
作者机构： 1.Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China
2.Bayingolin Mongolian Autonomous Prefecture Agr Sci, Korla 841000, Peoples R China
3.Xinjiang Acad Agr Sci, Res Inst Econ Crops, Urumqi 830091, Peoples R China
关键词：
EPF/EPFL; Gene family analysis; Cotton; Drought stress;
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2025 年 25 卷 1 期
页码：
收录情况： SCI
摘要： The development of stomatal lineage cells in terrestrial plants is tightly regulated by epidermal patterning factors (EPFs/EPFLs) and their downstream receptors, including ERECTA, TOO MANY MOUTHS (TMM), and SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs). These components form co-receptor complexes that activate the MAPK signaling cascade, playing critical roles in stomatal development, stress responses, and signal transduction. However, the EPF-ERECTA-TMM signaling network remains largely unexplored in cotton (Gossypium spp.). In this study, we performed a genome-wide identification and characterization of the EPF/EPFL, ERECTA, TMM, and SERK gene families in four cotton species (G. hirsutum, G. barbadense, G. arboreum, and G. raimondii), identifying 135 EPF/EPFL, 18 ERECTA, 6 TMM, and 90 SERK genes. Bioinformatics analyses-including gene collinearity, protein domain structure, cis-regulatory elements, and protein-protein interaction predictions-revealed functional divergence and stress-related regulatory potential across these families. Expression profiling in G. hirsutum indicated that several candidate genes, such as GhEPFL1-1, GhER1, and GhSERK17, are responsive to abiotic stresses. To validate these computational predictions, functional assays were conducted. Virus-induced gene silencing (VIGS) of GhEPFL1-1, GhER1, and GhSERK17 led to increased stomatal density and reduced drought tolerance, confirming their roles in stress adaptation. Furthermore, luciferase complementation imaging in Nicotiana benthamiana demonstrated direct interactions between GhEPFL1-1 and GhER1, and co-receptor complex formation with GhSERK17, consistent with molecular docking simulations. Collectively, this study lays a theoretical foundation for further exploration of the EPF/EPFL-mediated peptide-receptor signaling pathway in cotton and its potential application in breeding for stress resilience.
- 相关文献
作者其他论文 更多>>
-
Photosynthetic mechanism of cotton under fluctuating light field planted with different densities
作者：Lei, Zhangying;Li, Xilin;Zhang, Tong;Li, Xiaoming;He, Daohua;Li, Youzhong;Yang, Yanlong;Zhang, Yali
关键词：Plant density; Light intensity; Frequence of fluctuation light; Photosynthesis; Biomass
-
Comparative Transcriptomic Analysis of Gossypium hirsutum Fiber Development in Mutant Materials (xin w 139) Provides New Insights into Cotton Fiber Development
作者：Li, Chunping;Liu, Zhongshan;Yang, Yanlong;Lai, Chengxia;Ma, Jun;Aierxi, Alifu;Zhao, Jieyin
关键词：Gossypium hirsutum; mutant; fiber development; RNA-seq; candidate genes
-
GbPP2C80 Interacts with GbWAKL14 to Negatively Co-Regulate Resistance to Fusarium and Verticillium wilt via MPK3 and ROS Signaling in Sea Island Cotton
作者：Zhao, Nan;Guo, Anhui;Li, Bin;Jiang, Kaiyun;Wang, Baoliang;Adjibolosoo, Daniel;Xia, Zhanghao;Li, Huijing;Cui, Yanan;Hua, Jinping;Wang, Weiran;Wang, Meng;Zhou, Zixin;Aierxi, Alifu;Kong, Jie
关键词：Fusarium wilt; reactive oxygen species; Sea Island cotton; Verticillium wilt; wall-associated receptor kinase-like 14
-
Comparative Transcriptome Analysis of Gossypium hirsutum Mutant (xin w 139) and Wild-Type (Xin W 139) Plants During Seed Embryo Development
作者：Zhao, Jieyin;Li, Chunping;Yang, Yanlong;Ma, Jun;Lai, Chengxia;Tian, Liwen;Zhao, Jieyin;Maimaiti, Paerhati
关键词：
Gossypium hirsutum ; mutants; seed embryo development; RNA-seq; candidate genes -
Genome-Wide Identification and Preliminary Functional Analysis of BAM (β-Amylase) Gene Family in Upland Cotton
作者：Yang, Yanlong;Yusuyin, Mayila;Lai, Chengxia;Li, Chunping;Ma, Jun;Yang, Yanlong;Wang, Penglong;Kuerban, Wumaierjiang;Sun, Fenglei;Xiao, Fei
关键词：beta-amylase (BAM) gene family; upland cotton; genome-wide identification; fiber development; starch metabolism
-
Genome-wide association analysis revealed genetic variation and candidate genes associated with the yield traits of upland cotton under drought conditions
作者：Sun, Fenglei;Sun, Fenglei;Ma, Jun;Shi, Weijun;Yang, Yanlong
关键词：upland cotton; drought tolerance; genome-wide associated study; SNPs; candidate genes
-
Genome-wide Identification of the Upland Cotton (Gossypium hirsutum L.) B-BOX gene Family and its Expression After Drought Stress
作者：Sun, Fenglei;Sun, Fenglei;Ma, Jun;Yang, Yanlong;Wang, Penglong
关键词：B-BOX gene; Upland cotton; Gene expression; Drought resistance; qRT-PCR
