Genomic Analysis and Expression Dynamics of GH3 Genes Under Abiotic Stresses in Brassica rapa
文献类型： 外文期刊
作者： Karamat, Umer 1 ; Awan, Muhammad Jawad Akbar 2 ; Ahmad, Muhammad 3 ; Farooq, Muhammad Awais 2 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou, Peoples R China
2.Natl Inst Biotechnol & Genet Engn NIBGE, Constituent Coll Pakistan Inst Engn & Appl Sci, Agr Biotechnol Div, Faisalabad, Pakistan
3.China Agr Univ, Coll Vegetables Sci, Dept Hort, Beijing, Peoples R China
4.Univ Bologna, Dept Agr & Food Sci Alma Mater Studiorum, Bologna, Italy
关键词： abiotic stresses; < fixed-case > Brassica rapa ; BrGH3; genome wide characterization; phylogenetics; phytohormone
期刊名称：PLANT BREEDING （ 影响因子：1.8； 五年影响因子：2.0 ）
ISSN： 0179-9541
年卷期： 2025 年 144 卷 1 期
页码：
收录情况： SCI
摘要： In silico characterization of the Gretchen Hagen3 (GH3) gene family is required for understanding phytohormone regulation and stress responses in Brassica rapa to develop the improved stress-tolerant cultivars. The main objective of this research was to identify the key BrGH3 genes under different abiotic stresses. Using bioinformatics tools, a total of 27 BrGH3 genes were identified in B. rapa for which phylogenetic relationship, syntenic pairing, conserved motifs, miRNA regulation, and cis-regulatory elements of BrGH3s were also studied. BrGH3 gene expression has been examined in numerous tissues and under various abiotic and phytohormone stressors. Phylogenetic studies separated BrGH3 genes into three major classes and nine subclasses based on gene functional evolution. Collinearity analysis demonstrated that all BrGH3 genes had syntenic linkage with AtGH3, BnaGH3, and BolGH3, indicating that genome duplication mechanisms were crucial to BrGH3 gene evolution. The expression dynamics of BrGH3 under diverse phytohormonal stresses methyl jasmonate (MeJA), auxin (IAA), abscisic acid (ABA), gibberellic acid (GA), and salicylic acid (SA) at different time intervals suggest that BrGH3-5.1 has a consistently greater expression than other BrGH3 genes. Under salt, drought, and low temperature stress, BrGH3-1 and BrGH3-4 genes expressed comparatively higher. The findings suggest that five genes (BrGH3-5.1, BrGH3-5.2, BrGH3-15, BrGH3-17.2, and BrGH3-19) have a key role in inducing broad stress resilience. These results can be beneficial in developing stress-tolerant varieties of Chinese cabbage.
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