文献类型： 外文期刊

作者： Du, Wenchao ¹ ; Karamat, Umer ³ ; Cao, Liuqing ¹ ; Li, Yunpeng ² ; Li, Haili ¹ ; Li, Haoxin ¹ ; Wei, Lai ² ; Yang, Dongchen ⁴ ; Xia, Meng ¹ ; Li, Qiang ² ; Chen, Xueping ² ;

作者机构： 1.Baoding Univ, Biochem & Environm Engn Coll, Baoding 071000, Peoples R China

2.Hebei Agr Univ, Coll Hort, Collaborat Innovat Ctr Vegetable Ind Hebei, Key Lab Vegetable Germplasm Enhancement & Utilizat, Baoding 071000, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510642, Peoples R China

4.Hebei Agr Univ, Coll Plant Protect, Baoding 071001, Peoples R China

关键词： expression profiling; bioinformatics tools; TIR1/AFB gene family; picloram; stresses; VIGS

期刊名称：AGRONOMY-BASEL （ 影响因子：3.3； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 14 卷 7 期

页码：

收录情况： SCI

摘要： TIR1/AFB proteins are a class of auxin receptors with key roles in plant development and biotic and abiotic stress responses; several have been identified as targets of the auxin-mimicking herbicide picloram. In this study, we identified five putative TIR1/AFB gene family members in the important vegetable crop Solanum melongena (eggplant) and characterized them using bioinformatics tools and gene expression analyses. Phylogenetic analysis of the TIR1/AFBs classified them into three subgroups based on their Arabidopsis and Solanum lycopersicum homologs. AFB6 homologs were present only in S. melongena and S. lycopersicum, whereas AFB2/3 homologs were found only in Arabidopsis. One pair of S. melongena TIR1 homologs were located in syntenic regions in the genome and appeared to have arisen by segmental duplication. Promoter analysis revealed 898 cis-elements in the TIR1/AFB promoters, 125 of which were related to hormones, stress, light, or growth responses, but only SmAFB5 had a cis-acting regulatory element involved in auxin responsiveness (AuxRR-core). RNA sequencing and expression profiling showed that the TIR1/AFB genes were differentially expressed at different growth stages and in response to light, temperature, and drought. Only SmTIR1A expression was significantly induced by picloram treatment and different growth stages. TIR1/AFB expression is regulated by microRNAs (miRNAs) in other plant species, and we identified 6 or 29 miRNAs that potentially targeted the five TIR1/AFB genes on the basis of comparisons with S. lycopersicum and S. tuberosum miRNAs, respectively. Three-dimensional protein structure predictions revealed that all the TIR1/AFB proteins were very similar in structure, differing only in the numbers of alpha helices and in one angle linking an alpha helix and a beta sheet. For measuring the function of TIR1/AFB genes in response to drought, SmAFB5 was selected, and knockdown by virus-induced gene silence (VIGS) 35S::SmAFB5 lines showed resistance to drought compared to controls. These analyses provide insight into the potential functions of TIR1/AFBs during growth and in response to stress; they highlight differences among the SmTIR1/AFBs that may be useful for eggplant breeding.