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In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor

文献类型： 外文期刊

作者： Tang, Lingjuan 1 ; Zhang, Zhiyao 1 ; Sun, Ling 1 ; Gao, Xu 3 ; Zhao, Xinyue 1 ; Chen, Xinru 1 ; Zhu, Xingyu 1 ; Li, Aixue 4 ; Sun, Lijun 1 ;

作者机构： 1.Nantong Univ, Sch Life Sci, Nantong 226019, Jiangsu, Peoples R China

2.Nantong Univ, Anal & Testing Ctr, Nantong 226019, Jiangsu, Peoples R China

3.East China Univ Technol, Sch Chem & Mat Sci, Nanchang 330013, Jiangxi, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Res Ctr Intelligent Equipment, Beijing 100097, Peoples R China

关键词： abscisic acid; stainless steel sheet; electrochemicalimmunolectrodes; in situ electrochemical detection

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.7； 五年影响因子：6.0 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 31 期

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) plays an important regulatory role in plants. It is very critical to obtain the dynamic changes of ABA in situ for botanical research. Herein, coupled with paper-based analysis devices, electrochemical immunoelectrodes based on disposable stainless steels sheet were developed for ABA detection in plants in situ. The stainless steel sheets were modified with carbon cement, ferrocene-graphene oxide-multi walled carbon nanotubes nanocomposites, and ABA antibodies. The system can detect the ABA in the range of 1 nM to 100 mu M, with a limit of detection of 100 pM. The ABA content in tomato leaves under high salinity was detected in situ. The trend of ABA changes was similar to the expression of SlNCED1 and SlNCED2. Overall, this study offers an approach for in situ detection of ABA in plants, which will help to study the regulation mechanism of ABA in plants and to promote the development of precision agriculture.

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