文献类型： 外文期刊

作者： Liu, Gang-Shuai ¹ ; Huang, Hua ² ; Grierson, Donald ⁴ ; Gao, Ying ⁴ ; Ji, Xiang ¹ ; Peng, Zhen-Zhen ¹ ; Li, Hong-Li ¹ ; Niu, Xiao-Lin ¹ ; Jia, Wen ¹ ; He, Jian-Lin ¹ ; Xiang, Lan-Ting ¹ ; Gao, Hai-Yan ⁷ ; Qu, Gui-Qin ¹ ; Zhu, Hong-Liang ¹ ; Zhu, Ben-Zhong ¹ ; Luo, Yun-Bo ¹ ; Fu, Da-Qi ¹ ;

作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Fruit Biol, Beijing 100083, Peoples R China

2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China

4.Zhejiang Univ, Lab Fruit Qual Biol, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Peoples R China

5.Univ Nottingham, Sch Biosci, Plant Sci Div, Sutton Bonington Campus, Loughborough LE12 5RD, England

6.Chongqing Univ, Sch Life Sci, Key Lab Plant Hormones & Dev Regulat Chongqing, Chongqing 401331, Peoples R China

7.Zhejiang Acad Agr Sci, Food Sci Inst, Key Lab Postharvest Handing Fruits, Key Lab Fruits & Vegetables Postharvest & Proc Tec, Hangzhou 310021, Peoples R China

关键词： Cuticle; cutin; SlNOR-like1; tomato fruit; transcription factor; wax

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The plant cuticle is an important protective barrier on the plant surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although the pathway of plant cuticle biosynthesis has been clarified, knowledge of the transcriptional regulation network underlying fruit cuticle formation remains limited. In the present work, we discovered that tomato fruits of the NAC transcription factor SlNOR-like1 knockout mutants (nor-like1) produced by CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] displayed reduced cutin deposition and cuticle thickness, with a microcracking phenotype, while wax accumulation was promoted. Further research revealed that SlNOR-like1 promotes cutin deposition by binding to the promoters of glycerol-3-phosphate acyltransferase6 (SlGPAT6; a key gene for cutin monomer formation) and CUTIN DEFICIENT2 (SlCD2; a positive regulator of cutin production) to activate their expression. Meanwhile, SlNOR-like1 inhibits wax accumulation, acting as a transcriptional repressor by targeting wax biosynthesis, and transport-related genes 3-ketoacyl-CoA synthase1 (SlKCS1), ECERIFERUM 1-2 (SlCER1-2), SlWAX2, and glycosylphosphatidylinositol-anchored lipid transfer protein 1-like (SlLTPG1-like). In conclusion, SlNOR-like1 executes a dual regulatory effect on tomato fruit cuticle development. Our results provide a new model for the transcriptional regulation of fruit cuticle formation. In tomato fruit, SlNOR-like1 promotes cutin deposition by targeting and activating SlGPAT6 and SlCD2 . Meanwhile, SlNOR-like1 inhibits wax accumulation by targeting and inhibiting SlKCS1 , SlCER1-2 , SlWAX2 , and SlLTPG1-like .