UBC18 E2 conjugating enzyme depends on SINAT1 E3 ligase to destabilize the ESCRT component FREE1 in plant iron deficiency responses
文献类型： 外文期刊
作者： Liu, Chuanliang 1 ; Zhang, Tianrui 1 ; Liu, Weijie 1 ; Xiao, Zhidan 1 ; Yang, Chao 1 ; Peng, Changlian 1 ; Gao, Caiji 1 ; Shen, Wenjin 1 ; Li, Hongbo 1 ;
作者机构： 1.South China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China
4.Guangdong Prov Key Lab Sci & Technol Res Fruit Tre, Guangzhou 510640, Peoples R China
5.Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Imp, Guangzhou 510650, Peoples R China
6.Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Peoples R China
关键词： Arabidopsis; E2 ubiquitin-conjugating enzyme; UBC18; FREE1; stability; iron deficiency
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2024 年 120 卷 5 期
页码：
收录情况： SCI
摘要： E2 ubiquitin-conjugating enzymes play a crucial role in the ubiquitination process by catalyzing ubiquitin transfer. Although the function of ubiquitin-protein ligases (E3s) in plants response to diverse abiotic stress by targeting specific substrates has been well studied, the involvement of E2s in environmental responses and their downstream targets are not well understood. In this study, we demonstrated that the E2 ubiquitin-conjugating enzyme 18 (UBC18) influences the stability of FREE1 to modulate iron deficiency stress. UBC18 affects the ubiquitination of FREE1 and promotes its degradation, and overexpression of UBC18 decreases plants' sensitivity to iron deficiency by reducing FREE1 level, whereas the ubc18 mutant exhibits sensitivity due to elevated FREE1 accumulation. This study also identified that lysine residues K227, K295, K315, and K540 are required for FREE1 ubiquitination and stability regulation. Mutating these lysine residues in FREE1 resulted in plants' sensitivity to iron starvation. Taken together, our findings shed light on the mechanism of UBC18 in responding to iron deficiency stress by modulating the abundance of FREE1, and further elucidate the role of ubiquitination sites in FREE1 stability regulation and the plant iron deficiency response.
- 相关文献
作者其他论文 更多>>
-
SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress
作者：Huang, Ruihua;Wen, Mengrui;Feng, Bojin;Zhong, Xiaoqing;Yang, Yifeng;Liu, Minghui;Li, Hongqing;Yang, Chengwei;Peng, Changlian;Zhang, Shengchun;Wu, Pingzhi
关键词：
-
Pretreatment with nano-silver extends the post-harvest longevity of gladiolus cut flowers by reducing free water mobility
作者：Li, Xiumei;Liu, Qinjian;Chen, Yuanyuan;Chen, Zhongjian;Liu, Jun;Yan, Shijuan;Li, Hongbo;He, Shenggen
关键词：Water state; Nanoparticles; Vase life; Nuclear magnetic resonance; Cut flowers
-
Metabolomics Unveiled the Accumulation Characteristics of Taste Compounds During the Development and Maturation of Litchi Fruit
作者：Jiang, Nonghui;Liu, Wei;Xiao, Zhidan;Xiang, Xu;Zhong, Yun
关键词：litchi; metabolomics; taste compounds; development; mature
-
Metabolome and Transcriptome Analysis Provide Insights into Flower Bud Color Variation in the Adaptation to UV-B Radiation of Litchi
作者：Xiao, Zhidan;Wang, Jing;Jiang, Nonghui;Xiang, Xu;Liu, Wei
关键词：Litchi chinensis Sonn.; flavonoid biosynthesis; transcriptome; metabolome; UV-B; flower color variation
-
Genome-Wide Identification of SnRK1 Catalytic alpha Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses
作者：Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Wang, Hongxia;Wang, Jihua;Yuan, Ling;Wang, Ying
关键词：SnRK1; FLZ; Glycyrrhiza inflata; stress response; growth
-
Genome-Wide Development of Polymorphic SNP Markers and Evaluation of Genetic Diversity of Litchi (Litchi chinensis Sonn.)
作者：Liu, Wei;Xiao, Zhidan;Jiang, Nonghui;Fan, Chao;Xiang, Xu
关键词：litchi; germplasm resources; single-nucleotide polymorphism; genetic diversity; population structure
-
An LcMYB111-LcHY5 Module Differentially Activates an LcFLS Promoter in Different Litchi Cultivars
作者：Xiao, Zhidan;Wang, Jing;Jiang, Nonghui;Fan, Chao;Xiang, Xu;Liu, Wei
关键词：LcFLS; flower color; flavonol; LcMYB111; LcHY5
