文献类型： 外文期刊

作者： Xu, Zhongping ¹ ; Wang, Guanying ¹ ; Luo, Jing ² ; Zhu, Mingju ¹ ; Hu, Lisong ³ ; Liang, Sijia ⁴ ; Li, Bo ⁵ ; Huang, Xingxing ⁶ ; Wang, Ying ¹ ; Zhang, Guangyu ¹ ; Zhang, Can ¹ ; Zhou, Yi ¹ ; Yuan, Daojun ¹ ; Chen, Taiyu ¹ ; Chen, Lizhen ⁶ ; Ma, Weihua ¹ ; Gao, Wei ⁷ ; Lindsey, Keith ⁸ ; Zhang, Xianlong ¹ ; Ding, Fang ⁹ ; Jin, Shuangxia ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan, Hubei, Peoples R China

2.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Hubei, Peoples R China

3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Hainan, Peoples R China

4.Huanghuai Univ, Acad Ind Innovat & Dev, Zhumadian, Henan, Peoples R China

5.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol, Urumqi, Xinjiang, Peoples R China

6.Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pest, Wuhan, Hubei, Peoples R China

7.Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Henan Key Lab Plant Stress Biol, Kaifeng, Henan, Peoples R China

8.Univ Durham, Dept Biosci, Durham DH1 3LE, England

9.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Key Lab Plant Pathol, Wuhan, Hubei, Peoples R China

关键词： Adelphocoris suturalis; Chromosome-scale reference genome; Candidate effectors; Omnivory; Detoxification

期刊名称：BMC BIOLOGY （ 影响因子：5.4； 五年影响因子：7.1 ）

ISSN：

年卷期： 2023 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Adelphocoris suturalis (Hemiptera: Miridae) is a notorious agricultural pest, which causes serious economic losses to a diverse range of agricultural crops around the world. The poor understanding of its genomic characteristics has seriously hindered the establishment of sustainable and environment-friendly agricultural pest management through biotechnology and biological insecticides. Results Here, we report a chromosome-level assembled genome of A. suturalis by integrating Illumina short reads, PacBio, 10x Chromium, and Hi-C mapping technologies. The resulting 1.29 Gb assembly contains twelve chromosomal pseudomolecules with an N50 of 1.4 and 120.6 Mb for the contigs and scaffolds, respectively, and carries 20,010 protein-coding genes. The considerable size of the A. suturalis genome is predominantly attributed to a high amount of retrotransposons, especially long interspersed nuclear elements (LINEs). Transcriptomic and phylogenetic analyses suggest that A. suturalis-specific candidate effectors, and expansion and expression of gene families associated with omnivory, insecticide resistance and reproductive characteristics, such as digestion, detoxification, chemosensory receptors and long-distance migration likely contribute to its strong environmental adaptability and ability to damage crops. Additionally, 19 highly credible effector candidates were identified and transiently overexpressed in Nicotiana benthamiana for functional assays and potential targeting for insect resistance genetic engineering. Conclusions The high-quality genome of A. suturalis provides an important genomic landscape for further investigations into the mechanisms of omnivory, insecticide resistance and survival adaptation, and for the development of integrated management strategies.