Comprehensive investigation of the C2H2-type zinc finger protein transcription factor family in Aquilaria sinensis reveals the involvement of AsZFP9 in sesquiterpene biosynthesis
文献类型： 外文期刊
作者： Li, Yuji 1 ; Guo, Dong 1 ; Wang, Hao 1 ; Wang, Ying 1 ; Peng, Shiqing 1 ; Dai, Haofu 1 ; Mei, Wenli 1 ; Zhu, Jiahong 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Inst Trop Agr Resources, Inst Trop Biosci & Biotechnol, Key Lab Res & Dev Nat Prod Li Folk Med Hainan Prov, Haikou 571101, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572000, Peoples R China
3.Hainan Engn Res Ctr Agarwood, Haikou 571101, Peoples R China
关键词： Aquilaria sinensis; Zinc finger protein; Agarwood; Sesquiterpene biosynthesis
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 318 卷
页码：
收录情况： SCI
摘要： Agarwood, a valuable resin produced by Aquilaria trees in response to environmental stresses, is characterized by its rich sesquiterpene content. However, the regulatory mechanisms underlying the sesquiterpene biosynthesis remain poorly understood. C2H2-type zinc finger proteins (C2H2-ZFPs) are key transcriptional regulators involved in plant growth, stress responses, and secondary metabolism. In this study, we identified 56 AsC2H2ZFP genes in Aquilaria sinensis and systematically analyzed their subcellular localization, evolutionary relationships, conserved motifs and gene structures. Transcriptomic analysis revealed differential expression of 12 AsC2H2-ZFPs under agarwood inducer treatment, with their expression patterns significantly correlated with sesquiterpene biosynthesis-related genes. Notably, AsZFP9 was characterized as a nuclear-localized transcriptional activator, whose expression was markedly induced by wound and methyl jasmonate treatments. Transient overexpression of AsZFP9 in the stems of A. sinensis significantly increased sesquiterpene production and upregulated key biosynthetic genes. Investigations utilizing yeast one-hybrid, electrophoretic mobility shift assays, and dual-luciferase assays demonstrated that AsZFP9 directly binds to and activates the promoters of AsHMGR1 and AsTPS1, two critical genes in sesquiterpene biosynthesis. Our findings provide novel insights into the role of C2H2-ZFPs in A. sinensis and establish AsZFP9 as a key regulator of sesquiterpene accumulation and agarwood formation.
- 相关文献
作者其他论文 更多>>
-
Syntaxin 17 Translocation Mediated Mitophagy Switching Drives Hyperglycemia-Induced Vascular Injury
作者：Luo, Anqi;Liu, Xiaoquan;Liu, Haochen;Wang, Rui;Zheng, Yunsi;Gong, Jingwen;Wang, Shuting;Yun, Chuan;Chen, Zongcun;Jiang, Yanan;Dai, Haofu
关键词：(diabetes; Fis1; mitophagy; Syntaxin 17); vascular endothelial injury
-
Identification of targets and comparative study of administration methods for the lipid-lowering effects of fucoidan from Saccharina japonica
作者：Chen, Pengwei;Wang, Hao;Chen, Huiqin;Dai, Haofu;Hao, Li;Li, Quancai;Zhang, Miao;Jiang, Hongfei;Zhang, Renshuai;Jia, Ang
关键词：Fucoidan; Lipid-lowering activity; Dietary supplementation; Intestinal target; Gut microbiota
-
Comprehensive analysis of morphology, transcriptomics, and metabolomics of banana (Musa spp.) molecular mechanisms related to plant height
作者：Wei, Junya;Cheng, Shimin;Huang, Lina;Wei, Shouxing;Liu, Guoyin;Sun, Mingzhen;Wang, Hao;Liu, Debing;Yang, Ping
关键词：banana; metabolomics; transcriptomics; hormones; height
-
2-(2-Phenylethyl)chromone-Sesquiterpene Hybrids from Agarwood of Aquilaria sinensis: Characterization and Biological Activity Evaluation
作者：Xu, Guan-Hua;Liu, Shou-Bai;Xu, Guan-Hua;Wang, Ya-Li;Wang, Hao;Chen, Hui-Qin;Dong, Wen-Hua;Huang, Sheng-Zhuo;Cai, Cai-Hong;Yuan, Jing-Zhe;Mei, Wen-Li;Dai, Hao-Fu
关键词：Hainan agarwood; 2-(2-phenylethyl)chromone-sesquiterpene hybrids; structure elucidation; anti-inflammatory activity; neuroprotective activity; cytotoxic activity
-
Three new dimeric 2-(2-phenethyl)chromones from the agarwood of Aquilaria malaccensis
作者：Yan, Xian-Yun;Liang, Lin-Fu;Yan, Xian-Yun;Li, Wei;Wang, Hao;Dong, Wen-Hua;Zeng, Jun;Wu, Fei;Mei, Wen-Li;Dai, Hao-Fu;Yan, Xian-Yun;Li, Wei;Wang, Hao;Dong, Wen-Hua;Zeng, Jun;Wu, Fei;Mei, Wen-Li;Dai, Hao-Fu
关键词：Agarwood; Aquilaria malaccensis; 2-(2-phenethyl)chromone dimers; Anti-inflammatory activity
-
Hierarchical hybrid of tungsten nitride nanosheets integrated with nitrogen-doped carbon for efficiently electrocatalytical nitrogen fixation
作者：Wang, Jing;Hui, Liufu;Chen, Jing;Chen, Milin;Huang, Ziyi;Ma, Lin;Wang, Xian;Xi, Yanjie;Gao, Yihan;Wang, Ying;Zhang, Liying;Chen, Jing;Wang, Xian
关键词：Tungsten nitride; Hierarchical structure; Nitrogen-doped carbon; Nitrogen reduction reaction; Electrocatalyst
-
Potential influence of supplemental nutrients intake by adults on the development, fecundity, and population growth of Megalurothrips usitatus (Thysanoptera: Thripidae) offspring
作者：Li, Tiantian;Liu, Ying;Liang, Xiao;Wu, Chunling;An, Xingkui;Wang, Ying;Hao, Guifeng;Chen, Yiting;Chen, Qing;Liu, Ying;Liang, Xiao;Wu, Chunling;An, Xingkui;Wang, Ying;Hao, Guifeng;Chen, Yiting;Chen, Qing;Liu, Ying;Chen, Qing
关键词：population dynamics; nutritional ecology; leguminous crops; rearing method
