Host Gut-Derived Probiotic, Exiguobacterium acetylicum G1-33, Improves Growth, Immunity, and Resistance to Vibrio harveyi in Hybrid Grouper (Epinephelus fuscoguttatus ♀ x Epinephelus lanceolatus ♂)
文献类型： 外文期刊
作者： Zhang, Mingqing 1 ; Feng, Yuwei 1 ; Zhong, Zhongxuan 1 ; Du, Qianping 1 ; Yu, Wei 3 ; Wu, Jinhui 5 ; Huang, Xiaolin 3 ; Huang, Zhong 3 ; Xie, Guangting 1 ; Shu, Hu 1 ;
作者机构： 1.Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China
2.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China
3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Shenzhen Base, Shenzhen 518121, Peoples R China
4.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China
5.Agrotech Extens Ctr Guangdong Prov, Guangzhou 510500, Peoples R China
关键词： hybrid grouper; Exiguobacterium acetylicum; growth; immunity; mariculture
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2024 年 12 卷 8 期
页码：
收录情况： SCI
摘要： Several exogenous probiotics are applicable in fish culture; however, challenges in isolation and verification have hindered the full utilization of numerous host probiotics. Therefore, this study aimed to apply the host probiotic Exiguobacterium acetylicum G1-33 to hybrid grouper (Epinephelus fuscoguttatus female x Epinephelus lanceolatus male) cultures and explore its mechanism of action. In total, 360 hybrid grouper were divided into four groups, which were fed the following for 60 days: three received commercial feed with varying concentrations of E. acetylicum G1-33 (106, 108, and 1010 CFU/g), while a control group received commercial feed. The results showed that supplementation with 106 and 108 CFU/g of E. acetylicum G1-33 enhanced gut morphology, upregulated growth-related genes (ghr1, igf-2, s6k1, tor), and promoted growth, with supplementation with 108 CFU/g resulting in the most notable enhancement. However, supplementation with 1010 CFU/g inhibited growth, possibly because of changes in intestinal morphology. Additionally, supplementation with E. acetylicum G1-33 upregulated the expression of immune-related genes (c3, myd88, Cu/Zn-sod, tlr3, and tnf2) in the liver and head kidney but led to an increase in malondialdehyde content, as well as a decrease in alkaline phosphatase and acid phosphatase activities, in the liver and serum, indicating increased oxidative stress. Moreover, supplementation with 106 and 108 CFU/g E. acetylicum G1-33 enhanced the widespread expression of immune-related genes in the head kidney and liver, respectively, and improved resistance to Vibrio harveyi, whereas supplementation with 1010 CFU/g weakened this resistance. In conclusion, E. acetylicum G1-33, particularly at 108 CFU/g, emerged as an effective probiotic, optimizing growth performance and immunity in hybrid grouper. This research is pioneering in its application of E. acetylicum in mariculture, potentially broadening the range of probiotic strategies in aquaculture.
- 相关文献
作者其他论文 更多>>
-
Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of Litopenaeus vannamei
作者：Yan, Hailiang;Liang, Hong;Yan, Hailiang;Wang, Yun;Liang, Hong;Duan, Yafei;Wang, Jun;Zhou, Chuanpeng;Wang, Yun;Duan, Yafei;Wang, Jun;Zhou, Chuanpeng;Wang, Yun;Huang, Zhong
关键词：Litopenaeus vannamei; lysophospholipids; antioxidant capacity; digestion; intestinal microbiota
-
Effects of alginate oligosaccharides on the growth performance, immunity, and intestinal health of the hybrid grouper (Epinephelus fuscoguttatus♀ x Epinephelus lanceolatu ♂)
作者：An, Shengzhe;Lin, Ziyang;Han, Liqin;Zhou, Chuanpeng;Lin, Heizhao;Yu, Wei;An, Shengzhe;Han, Liqin;Lin, Heizhao;Yu, Wei;An, Shengzhe;Han, Liqin;Guo, Ran;Xun, Pengwei
关键词：Gut microbiome modulation; Immune enhancement; Optimal AOS supplementation
-
Chromosome-level genome assembly and annotation of the White-spotted spinefoot Siganus canaliculatus
作者：Huang, Xiaolin;Xian, Lin;Yang, Yukai;Zhang, Dianchang;Huang, Xiaolin;Xian, Lin;Yang, Yukai;Zhang, Dianchang;Huang, Xiaolin;Yang, Yukai;Zhang, Dianchang;Lu, Yanke;Zhang, Hui;Huang, Shiting;Wang, Lei;Li, Chao;Xian, Lin
关键词：
-
Integration of Transcriptomics and Metabolomics Reveals Mechanisms of High-Temperature Stress Tolerance in the Hepatopancreas of Penaeus monodon
作者：Liu, Li;Zhao, Peng;Yang, Lishi;Li, Yundong;Shi, Jianzhi;Liu, Li;Li, Yundong;Yang, Qibin;Huang, Zhong;Yang, Yukai;Chen, Yibiao;Huang, Jianhua
关键词：
Penaeus monodon ; high-temperature stress; energy supply; immune regulation; amino acid metabolism; glutathione metabolism -
Gut Microbiota Response to Experimental Acute Cold Stress in Juvenile Yellowfin Tuna (Thunnus albacares)
作者：Huang, Junhua;Fu, Zhengyi;Yu, Wei;Ma, Zhenhua;Wu, Jinhui;Huang, Junhua;Fu, Zhengyi;Yu, Wei;Ma, Zhenhua;Huang, Junhua;Fu, Zhengyi;Yu, Wei;Ma, Zhenhua;Huang, Junhua;Fu, Zhengyi;Yu, Wei;Ma, Zhenhua;Huang, Junhua;Fu, Zhengyi;Ma, Zhenhua;Hou, Bowen;Zhang, Tao
关键词：yellowfin tuna; low-temperature stress; gut microbiota; alpha diversity; species composition
-
Effects of Eucalyptus Essential Oil on Growth, Immunological Indicators, Disease Resistance, Intestinal Morphology and Gut Microbiota in Trachinotus ovatus
作者：Lin, Ziyang;Chen, Yaqi;Gao, Zhenchuang;Xun, Pengwei;Lin, Ziyang;Zhou, Chuanpeng;Yu, Wei;Lin, Ziyang;An, Shengzhe;Zhou, Chuanpeng;Feng, Juan;Lin, Heizhao;Yu, Wei;Lin, Heizhao;Yu, Wei
关键词：eucalyptus essential oil; immunological parameters; intestinal microflora; disease resistance; Trachinotus ovatus
-
The Impact of Varying Spatiotemporal Scales on Different Joint Species Distribution Models: A Case Study of Pelagic Fish Species in the Northwest Pacific Ocean
作者：Fang, Xingnan;Zhang, Ping;Xing, Qinwang;Chen, Xinjun;Yu, Wei;Chen, Xinjun;Chen, Xinjun;Yu, Wei;Chen, Xinjun;Yu, Wei;Chen, Xinjun;Yu, Wei;Cao, Jie;Zhang, Heng
关键词：interspecies relationships; Joint Species Distribution Models (JSDMs); Northwest Pacific Ocean; pelagic fish; spatiotemporal scale
