Enzymatically synthesized gamma-[Glu]((n >= 1))-Gln as novel calcium-binding peptides to deliver calcium with enhanced bioavailability
文献类型： 外文期刊
作者： Xiang, Huan 1 ; Huang, Hui 1 ; Sun-Waterhouse, Dongxiao 2 ; Hu, Xiao 1 ; Li, Laihao 1 ; Waterhouse, Geoffrey I. N. 4 ; Tang, Runmei 2 ; Xiong, Jian 2 ; Cui, Chun 2 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural Affairs,Natl Res & Dev Ctr Aqu, Guangzhou 510300, Guangdong, Peoples R China
2.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
3.Res Inst Food Nutr & Human Hlth, Guangzhou 510640, Peoples R China
4.Univ Auckland, Sch Chem Sci, Private Bag 92019, Auckland, New Zealand
关键词： gamma-[Glu]((n >= 1))-Gln; gamma-[Glu]((n >= 1))-Gln-Ca chelate; Coordination mechanism; Caco-2 cells; Calcium bioavailability
期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）
ISSN： 0308-8146
年卷期： 2022 年 387 卷
页码：
收录情况： SCI
摘要： Novel gamma-[Glu]((n >= 1))-Gln-Ca chelates were prepared by using gamma-[Glu]((n >= 1))-Gln (mixture peptides containg gamma-Glu-Gln, gamma-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Glu-Glu-G1n) which were synthesized by glutaminase. Based on single factor and orthogonal array experiments, the optimal calcium-chelation conditions were as follows: 30 min chelation at 70 degrees C; peptide concentration, 0.15 g/mL; mixture peptides-tocalcium mass ratio, 25:1. The particle size of chelates was 743.6 +/-( )64.2 nm and the chelates appeared stable neutral molecular entities with 91.33% of chelation rate and 35.2 mg/g of calcium, also with higher thermostability. Chelated calcium ions might be surrounded by coordination bonds linking to two or more connecting points: oxygen atoms (carboxyl oxygen, hydroxyl oxygen and oxygen of peptide bonds) and nitrogen atoms (amino nitrogen). The chelates could be used for food/nutraceutical applications, as no toxicity at 0.125-10 mg/mL and enhanced efficiency in calcium release (releasing percentage over 85% at pH 8.0), transport and uptake compared with CaCl2 were detected in Caco-2 cells.
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