Isolation and identification of Chryseobacterium indologenes and its pathological changes in Pelodiscus sinensis
文献类型： 外文期刊
作者： Xing, Yangyang 1 ; Xue, Mingyang 2 ; Xiao, Zidong 2 ; Hu, Xiaowei 2 ; Zhai, Jiale 2 ; Zhang, Chunjie 2 ; Jiang, Nan 2 ; Fan, Yuding 2 ; Meng, Yan 2 ; Zhou, Yong 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
关键词： antibiotic susceptibility testing; Chryseobacterium indologenes; histopathology; pathogenicity; Pelodiscus sinensis
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.5； 五年影响因子：2.6 ）
ISSN： 0140-7775
年卷期： 2023 年
页码：
收录情况： SCI
摘要： The Chinese revered a species of aquatic reptile known as Pelodiscus sinensis as both an edible and medicinal species. When artificially breeding, many deaths occurred at the farmed P. sinensis, mainly due to excessive breeding density, water contamination, and turtles biting each other secondary to bacterial infections. In this study, an isolate of gram-negative bacteria WH0623 was isolated from the liver and kidney of diseased P. sinensis to trace the potential pathogen of this disease. Based on biochemical characteristics and 16S rRNA gene sequencing analyses, this isolated strain of WH0623 was identified as Chryseobacterium indologenes. The strain's median lethal dose (LD50) was 3.3 x 10(5) colony-forming units (CFU)/g per fish weight tested using artificial infection. Histopathological analysis revealed pathological changes, including cell swelling, hyperaemia, and necrosis in many tissues. Antibiotic susceptibility tests revealed that the bacteria WH0623 was susceptible to doxycycline, sulphonamides, ceftazidime, norfloxacin, and ciprofloxacin. These antibiotics could treat the disease. In conclusion, the pathogen causing the death of farmed P. sinensis was isolated and identified, and a drug-sensitive test was conducted. Our findings contribute to the future diagnosis and treatment of the disease.
- 相关文献
作者其他论文 更多>>
-
Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
作者：Xue, Mingyang;Xu, Chen;Huang, Zhenyu;Meng, Yan;Jiang, Nan;Fan, Yuding;Zhou, Yong;Xue, Mingyang
关键词：
Cyprinid herpesvirus 2 ; ORF41; pyruvate dehydrogenase -
Establishment and characterization of a hepatopancreas cell line from gibel carp (Carassius auratus gibelio) and its susceptibility to several fish viruses
作者：Duan, Qiudi;Ding, Huan;Ouyang, Lili;Ouyang, Shuang;Cao, Jiajia;Zhou, Jiezhi;Yuan, Ganjun;Wu, Xiaoyu;Pan, Xiaoyi;Li, Siqi;Tian, Yihan;Zhong, Qiwang;Fan, Yuding;Wu, Xiaoyu;Zhong, Qiwang
关键词：Hepatopancreas; Cell line; Chinese giant salamander iridovirus (GSIV); Spring viraemia of carp virus (SVCV); Gibel carp (Carassius auratus gibelio)
-
Identification of a novel circovirus associated with turbot (Scophthalmus maximus) acute hemorrhage disease
作者：Wang, Huilin;Huang, Zhihui;Li, Jie;Jiang, Jingzhe;Xu, Liming;Zhou, Yong;Qin, Qiwei;Wei, Jingguang;Wang, Qiyao;Xiao, Zhizhong;Li, Jie
关键词：Turbot; Turbot acute hemorrhage disease; Circovirus; Pathogen
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Viperin and Its Effect on SVCV Replication in Common Carp, Cyprinus carpio
作者：Meng, Yan;Hu, Xi;Jiang, Nan;Fan, Yuding;Li, Yiqun;Xue, Mingyang;Xu, Chen;Liu, Wenzhi;Zhou, Yong
关键词：common carp (
Cyprinus carpio );viperin ; characteristics; antiviral activities -
Immunological Responses and Protection in the Largemouth Bass (Microterus salmoides) Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV)
作者：Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Zhou, Yong;Yang, Tao;Li, Chenyang;Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Li, Yiqun;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Huang, Zhenyu;Zhou, Yong;Jiang, Nan
关键词：largemouth bass; largemouth bass ranavirus; inactivated vaccine; relative percent survival rates; immune response
-
A Multidrug-Resistant Escherichia coli Caused the Death of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
作者：Xue, Mingyang;Hu, Xiaowei;Jiang, Nan;Liu, Wei;Xiao, Zidong;Zhang, Chunjie;Fan, Yuding;Meng, Yan;Zhou, Yong;Xue, Mingyang;Wu, Yeying;Liang, Tianwang;Zhang, Huixuan
关键词：
Pelodiscus sinensis ;Escherichia coli ; multidrug-resistant; drug resistance genes
