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Identification of a novel circovirus associated with turbot (Scophthalmus maximus) acute hemorrhage disease

文献类型： 外文期刊

作者： Jiang, Jingzhe 2 ; Wang, Huilin 1 ; Xu, Liming 3 ; Zhou, Yong 4 ; Qin, Qiwei 5 ; Wang, Qiyao 6 ; Huang, Zhihui 1 ; Wei, Jingguang 5 ; Xiao, Zhizhong 7 ; Li, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Marine Sci & Technol Ctr,Yellow Sea Fisher, Qingdao 266071, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Dept Aquat Anim Dis & Control, Harbin 150070, Peoples R China

4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, 8 1st Wudayuan Rd, Wuhan 430223, Hubei, Peoples R China

5.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China

6.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Aquat Anim Dis, Minist Agr & Rural Affairs, Shanghai 200237, Peoples R China

7.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266003, Peoples R China

8.China Aquat Prod Proc & Mkt Alliance, Tutbot Branch, Beijing 100125, Peoples R China

关键词： Turbot; Turbot acute hemorrhage disease; Circovirus; Pathogen

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 595 卷

页码：

收录情况： SCI

摘要： Turbot (Scophthalmus maximus) is an economically important farming fish in China. However, an emerging disease named turbot acute hemorrhage disease (TAHD) has been affecting turbot farms since November 2019. TAHD is characterized by severe bleeding in the fish fins and may lead to significant mortality in one or two weeks, with accumulated mortality exceeding 90 %. The TAHD spread rapidly across the major turbot farming regions in China and resulted in significant economic losses. Virome sequencing was utilized in the diseased fish to discover a novel turbot circovirus (TCV). The TCV particle is similar to 30 nm in size and is located in the fish spleen and kidney. The TCV genome is 1774 bp long, with three open reading frames (ORFs) encoding the replication protein (Rep), capsid protein (Cap), and ORF3 of unknown function. The identity of the TCV Rep sequence was <53 % compared to the reported circovirus sequences. Phylogenetic analysis of the Rep and Cap protein sequences revealed that TCV is a novel circovirus. The polymerase chain reaction detection method was used to analyze the clinical TAHD samples from 2019, which were all TCV-positive. Various cell lines, including turbot kidney, epithelioma papilloma cyprinid, Chinook salmon embryo, and spotted halibut kidney, were used for TCV isolation. However, no cytopathic effect or replication was observed. The diseased fish tissue homogenate filtrate was used for experimental infection. As a result, all of the infected turbot showed signs of natural infection, with increasing numbers of cap gene copies post-infection. Based on these results, it was hypothesized that the novel circovirus TCV is closely associated with TAHD. Our research also indicated that the circovirus represents a significant threat to fish and more attention should be paid to it in aquaculture.

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