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Production and evaluation of a bivalent adjuvant inactivated vaccine against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus

文献类型： 外文期刊

作者： Zhang, Zhen 1 ; Guan, Chunyan 1 ; Zhao, Jingzhuang 1 ; Lin, Jingnan 4 ; Shao, Yizhi 1 ; Li, Linfang 1 ; Lu, Tongyan 1 ; Chen, Peng 5 ; Zhang, Yong-An 3 ; Xu, Liming 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Dept Aquat Anim Dis & Control, Harbin 150070, Peoples R China

2.Key Lab Aquat Anim Dis & Immune Technol Heilongjia, Harbin 150070, Peoples R China

3.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Hubei Hongshan Lab, Wuhan, Peoples R China

4.Ningbo Sansheng Biol Technol Co Ltd, Biopharmaceut R&D Dept, Ningbo 315012, Zhejiang, Peoples R China

5.Xinjiang Uygur Autonomous Reg Fisheries Res Inst, Urumqi 830000, Xinjiang, Peoples R China

关键词： Roller bottles; IHNV; IPNV; MontanideTM GEL 02PR; Bivalent inactivated vaccine

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 597 卷

页码：

收录情况： SCI

摘要： Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens to salmon and trout worldwide. In this study, we developed a process for producing IHNV and IPNV (genogroup 1, IPNV 1 and genogroup 5, IPNV 5) using EPC and CHSE-214, respectively, in 750 cm2 rolling bottles. With adjuvant of MontanideTM GEL 02PR, the monovalent vaccines made with produced IHNV, IPNV 1, or IPNV 5 provided strong immune protection against themselves in rainbow trout, RPS of 79.7 % to IHNV at 15 days post vaccination (dpv) and complete inhibition on virus load in vivo to IPNV 1 or IPNV 5 at 30 dpv. The weak cross-protection effect between IPNV 1 and IPNV 5 was characterized, with IPNV 1 providing higher crossinhibition rates than IPNV 5 when made into monovalent vaccines. Bivalent vaccines, composed of IHNV: IPNV 1: MontanideTM GEL 02PR in 2:7:1 (H2I7) and 3.5:5.5:1 (H3I5), effectively induced immune response and neutralizing antibodies to protect rainbow trout against IHNV, IPNV 1, and IPNV 5. Furthermore, H2I7 was recommended as the appropriate compatibility ratio due to its better performance in most aspects, RPS of 72.2 % and 54.5 % to IHNV at 44 dpv and 74 dpv respectively, along with higher inhibition rate to IPNV 1 and IPNV 5 at 44 and 74 dpv. This study provides references for the production and compatibility process of IHNV and IPNV bivalent inactivated vaccines.

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