Analysis of Transcriptome Differences Between Subcutaneous and Intramuscular Adipose Tissue of Tibetan Pigs
文献类型： 外文期刊
作者： Li, Xinming 1 ; Huang, Qiuyan 1 ; Meng, Fanming 1 ; Hong, Chun 1 ; Li, Baohong 1 ; Yang, Yecheng 1 ; Qu, Zixiao 1 ; Wu, Junda 1 ; Li, Fei 1 ; Xin, Haiyun 1 ; Hu, Bin 1 ; Wu, Jie 1 ; Hu, Chuanhuo 2 ; Zhu, Xiangxing 3 ; Tang, Dongsheng 3 ; Du, Zongliang 1 ; Wang, Sutian 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangdong Prov Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China
2.Guangxi Univ, Coll Anim Sci & Technol, Guangxi Key Lab Anim Breeding Dis Control & Preven, Nanning 530004, Peoples R China
3.Foshan Univ, Sch Med, Foshan 528000, Peoples R China
关键词： Tibetan pig; fat deposition; subcutaneous fat; intramuscular fat; RNA-seq
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 16 卷 3 期
页码：
收录情况： SCI
摘要： Background/Objectives: Fat deposition traits in pigs directly influence pork flavor, tenderness, and juiciness and are closely linked to overall pork quality. The Tibetan pig, an indigenous breed in China, not only possesses a high intramuscular fat content but also exhibits a unique fat metabolism pattern due to long-term adaptation to harsh environments. This makes it an excellent genetic and physiological model for investigating fat deposition characteristics. Adipose tissue from different body regions displays varying morphologies, cytokines, and adipokines. This study aimed to examine adipose tissue deposition characteristics in different parts of Tibetan pigs and provide additional data to explore the underlying mechanisms of differential fat deposition. Methods: Our research identified significant differences in the morphology and gene expression patterns between subcutaneous fat (abdominal fat [AF] and back fat [BF]) and intramuscular fat (IMF) in Tibetan pigs. Results: Histological observations revealed that subcutaneous fat cells were significantly larger in area and diameter compared to IMF cells. The transcriptomic analysis further identified differentially expressed genes (DEGs) between subcutaneous fat and IMF, with a total of 65 DEGs in BF vs. IMF and 347 DEGs in AF vs. IMF, including 25 DEGs common to both comparisons. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that these genes were significantly associated with lipid metabolism-related signaling pathways, such as the Wnt, mTOR, and PI3K-Akt signaling pathways. Several DEGs, including DDAH1, ADRA1B, SLCO3A1, and THBS3, may be linked to the differences in fat deposition in different parts of Tibetan pigs, thereby affecting meat quality and nutritional value. Conclusions: These findings provide new insights into the unique fat distribution and deposition characteristics of Tibetan pigs and establish a foundation for breeding strategies aimed at improving pork quality.
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