文献类型： 外文期刊

作者： Huang, Qiuyan ¹ ; Zhang, Kunli ² ; Meng, Fanming ¹ ; Lin, Sen ³ ; Hong, Chun ¹ ; Li, Xinming ¹ ; Li, Baohong ¹ ; Wu, Jie ¹ ; Xin, Haiyun ¹ ; Hu, Chuanhuo ⁴ ; Zhu, Xiangxing ⁵ ; Tang, Dongsheng ⁵ ; Pei, Yangli ⁶ ; Wang, Sutian ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangdong Prov Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov, Guangdong Prov Observat & Res Stn Anim Dis, Guangzhou 510640, Peoples R China

3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510640, Peoples R China

4.Guangxi Univ, Coll Anim Sci & Technol, Guangxi Key Lab Anim Breeding Dis Control & Preven, Nanning 530004, Peoples R China

5.Foshan Univ, Sch Med, Foshan 528051, Peoples R China

6.Foshan Univ, Guangdong Higher Educ Inst, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan 528225, Peoples R China

关键词： pigs; IMF; transcriptome; metabolome; fat deposition

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2025 年 15 卷 13 期

页码：

收录情况： SCI

摘要： The aim of this study is to investigate the physiological characteristics and regulatory mechanisms of porcine intramuscular fat (IMF), subcutaneous fat (take back fat (BF), for example), and visceral fat (take perienteric fat (PF), for example) to address the challenge of optimizing meat quality without excessive fat deposition. Many improved breed pigs have fast growth rates, high lean meat rates, and low subcutaneous fat deposits, but they also have low IMF content, resulting in poor meat quality. There is usually a positive correlation between intramuscular fat and subcutaneous fat deposits. This study selected eight-month-old female Tibetan pigs as experimental subjects. After slaughter, fat samples were collected. Histological differences in adipocyte morphology were observed via hematoxylin-eosin (HE) staining of tissue sections, and phenotypic characteristics of different adipose tissues were analyzed through fatty acid composition determination. Transcriptome sequencing and untargeted metabolomics were employed to perform pairwise comparisons between different fatty tissues to identify differentially expressed genes and metabolites. A siRNA interference model was constructed and combined with Oil Red O staining and lipid droplet optical density measurement to investigate the regulatory role of WNT16 in adipocyte differentiation. Comparative analysis of phenotypic and fatty acid composition differences in adipocytes from different locations revealed that IMF adipocytes have significantly smaller areas and diameters compared to other fat depots and contain higher levels of monounsaturated fatty acids. Integrated transcriptomic and metabolomic analyses identified differential expression of WNT16 and L-tyrosine, both of which are involved in the melanogenesis pathway. Functional validation showed that inhibiting WNT16 in porcine preadipocytes downregulated adipogenic regulators and reduced lipid droplet accumulation. This cross-level regulatory mechanism of "phenotype detection-multi-omics analysis-gene function research" highlighted WNT16 as a potential key regulator of site-specific fat deposition, providing new molecular targets for optimizing meat quality through nutritional regulation and genetic modification.