文献类型： 外文期刊

作者： Ruan, D. ¹ ; Fan, Q. L. ¹ ; Zhang, S. ¹ ; EI-Senousey, H. K. ² ; Fouadt, A. M. ² ; Lin, X. J. ¹ ; Dong, X. L. ¹ ; Deng, Y. F. ¹ ; Yan, S. J. ³ ; Zheng, C. T. ¹ ; Jiang, Z. Y. ¹ ; Jiang, S. Q. ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Key Lab Anim Nutr & Feed Sci South China, Inst Anim Sci,Minist Agri & Rural Affairs, Guangzhou 510640, Peoples R China

2.Cairo Univ, Fac Agr, Dept Anim Prod, Giza 12613, Egypt

3.CJ Int Trading Co Ltd, Shanghai 201107, Peoples R China

4.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China

关键词： isoleucine; yellow -feathered chickens; amino acid transporters; protein metabolism; gut microbiota

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 8 期

页码：

收录情况： SCI

摘要： This study investigated the effects of die-tary isoleucine (Ile) on growth performance, intestinal expression of amino acid transporters, protein metabo-lism-related genes and intestinal microbiota in starter phase Chinese yellow-feathered chickens. Female Xin-guang yellow-feathered chickens (n = 1,080, aged 1 d) were randomly distributed to 6 treatments, each with 6 replicates of 30 birds. Chickens were fed diets with 6 lev-els of total Ile (6.8, 7.6, 8.4, 9.2, 10.0, and 10.8 g/kg) for 30 d. The average daily gain and feed conversion ratio were improved with dietary Ile levels (P < 0.05). Plasma uric acid content and glutamic-oxalacetic transaminase activity were linearly and quadratically decreased with increasing dietary Ile inclusion (P < 0.05). Dietary Ile level had a linear (P < 0.05) or quadratic (P < 0.05) effect on the jejunal expression of ribosomal protein S6 kinase B1 and eukaryotic translation initiation factor 4E binding protein 1. The relative expression of jejunal 20S proteasome subunit C2 and ileal muscle ring finger -con-taining protein 1 decreased linearly (P < 0.05) and quadratically (P < 0.05) with increasing dietary Ile levels. Dietary Ile level had a linear (P = 0.069) or qua-dratic (P < 0.05) effect on the gene expression of solute carrier family 15 member 1 in jejunum and solute carrier family 7 member 1 in ileum. In addition, bacterial 16S rDNA full-length sequencing showed that dietary Ile increased the cecal abundances of the Firmicutes phy-lum, and Blautia, Lactobacillus, and unclassified_Lach-nospiraceae genera, while decreased that of Proteobacteria, Alistipes, and Shigella. Dietary Ile levels affected growth performance and modulated gut micro -biota in yellow-feathered chickens. The appropriate level of dietary Ile can upregulate the expression of intestinal protein synthesis-related protein kinase genes and con-comitantly inhibit the expression of proteolysis-related cathepsin genes.