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A Potential Role of the Translation Elongation Factor eef1a1 in Gonadal High-Temperature Perception in Chinese Tongue Sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Wang, Qian 1 ; Liu, Qian 2 ; Ma, Wenxiu 2 ; Wang, Rui 2 ; Li, Shuo 2 ; Dong, Zhongdian 1 ; Shao, Changwei 2 ;

作者机构： 1.Guangdong Ocean Univ, Fisheries Coll, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang 524000, Peoples R China

2.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Processes, Qingdao 266071, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

4.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Resistance Biol, Jinan 250014, Peoples R China

5.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China

6.Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524000, Peoples R China

关键词： eef1a1; high temperature; sexual response; sex differentiation; gonad; Cynoglossus semilaevis

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 13 期

页码：

收录情况： SCI

摘要： Simple Summary The eukaryotic elongation factor 1 alpha (eef1a) gene is vital for protein translation by delivering aminoacylated tRNAs to the A/P site of the ribosome via the GTP-dependent reaction. Here, the Chinese tongue sole (Cynoglossus semilaevis) eef1a1 gene was identified, and its potential role in gonadal high-temperature perception was assessed. The full-length sequence of eef1a1 cDNA was 1809 base pair (bp) encoding a putative protein of 461 amino acids. The expression levels of eef1a1 in the ovary were significantly higher than that in the testis from 6 mpf to 3 ypf. Under high-temperature induction during sex differentiation, eef1a1 was significantly down-regulated in males, while the difference was not detected in females. Furthermore, the rapid response of eef1a1 to environmental high temperature was assessed in vitro. Our findings suggest that C. semilaevis eef1a1 might be essential for the molecular response regulatory network of external temperature affecting internal sex differentiation. The eukaryotic translation elongation factor 1 alpha (eef1a) gene has a well-defined role in protein synthesis. However, its role in external temperature perception and internal sex differentiation and development is still unclear. In this study, eef1a1 was identified and functionally analyzed in Chinese tongue sole (Cynoglossus semilaevis). The eef1a1 cDNA, 1809 bp in length, had a 1386 bp open reading frame (ORF) that encoded a 461 amino acid polypeptide containing one EF-1_alpha domain. eef1a1 expression levels were investigated across different tissues and during gonadal development. In the gonad, eef1a1 showed a sexually dimorphic expression pattern with a statistically higher expression level in the ovary than in the testis from 6 months postfertilization to 3 years postfertilization. Under high temperature (28 degrees C) treatment during C. semilaevis sex differentiation (from 30 days postfertilization to 3 months postfertilization), eef1a1 was statistically down-regulated in males, while the difference was not detected in females. In addition, the dual-luciferase assay exhibited that eef1a1 can respond to high temperature rapidly. Based on these results, C. semilaevis eef1a1 might have a dual role in the perception of external temperature changes and sex differentiation regulation.

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