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Rapid pre-analysis derivatization-enabled high-throughput immunoassay for nitrite detection in foods

文献类型： 外文期刊

作者： Lin, Shi-Qi 1 ; Deng, Hao 1 ; Jia, Bao-Zhu 3 ; Chen, Zi-Jian 2 ; Hao, Fan-Yu 2 ; Yang, Si-Qi 2 ; Wang, Hong 2 ; Hongsibsong, Surat 4 ; Luo, Lin 2 ; Xu, Zhen-Lin 2 ;

作者机构： 1.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold Chain Hainan P, Haikou 571100, Peoples R China

2.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

3.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Peoples R China

4.Chiang Mai Univ, Res Inst Hlth Sci, Sch Hlth Sci Res, Chiang Mai 50200, Thailand

关键词： Nitrite; Hapten; Immunoassay; Monoclonal antibody

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2024 年 207 卷

页码：

收录情况： SCI

摘要： Nitrite is a well-known food additive and water pollutant that has garnered extensive attention due to both of its adverse and beneficial effects on human health, calling for rapid and reliable analytical methods. However, the simple and diminutive structure of nitrite has impeded the development of screening immunoassay for this substance. In this work, based on the rapid and selective derivatization of nitrite into benzotriazole (BTA) by ophenylenediamine, two haptens targeting the structure of BTA were designed and synthesized, and a specific monoclonal antibody against BTA was generated. Using this antibody, an indirect competitive enzyme-linked immunosorbent assay (ELISA), involving a rapid pre-analysis derivatization, was developed for the detection of nitrite in foods. The limits of detection in drinking water, pickle, and ham sausage were 2.0 mu g/L, 0.05 mg/kg, and 0.05 mg/kg, respectively. The recoveries ranged from 82.7% to 114.6%, and the results of this ELISA correlated well with those from the standard spectrophotometry method. This pioneering immunoassay for nitrite holds great promise for routine monitoring, ensuring food safety and consumer health.

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