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Transcriptomic Analysis Provides Insights into Anthocyanin Accumulation in Mulberry Fruits

文献类型： 外文期刊

作者： Mo, Rongli 1 ; Zhang, Na 1 ; Li, Jinxin 1 ; Jin, Qiang 2 ; Zhu, Zhixian 1 ; Dong, Zhaoxia 1 ; Li, Yong 1 ; Zhang, Cheng 1 ; Yu, Cui 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Econ Crops, Wuhan 430064, Peoples R China

2.Tarim Univ, Coll Hort & Forestry, Alar 843300, Peoples R China

关键词： mulberry fruit; anthocyanins biosynthetic pathway; RAN-seq; ethylene; MYB; bHLH; ERF

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 10 期

页码：

收录情况： SCI

摘要： Mulberry fruits are rich in anthocyanins, which are important secondary metabolites that give mulberries their bright color, favorable taste and high nutritional quality, making them a popular fruit. However, few studies have focused on the molecular mechanism underlying anthocyanin accumulation in mulberries and the gene regulatory networks of anthocyanin biosynthetic pathways remain largely unknown. In this study, we performed RNA sequencing to identify differentially expressed genes (DEGs) associated with anthocyanin accumulation between two mulberry genotypes ('Zi Jing', ZJ and 'Zhen Zhu Bai', ZZB, with purple and white fruit flesh, respectively) at 5, 18, 27 and 31 days after flower. Using transcriptome analysis, we explored several key DEGs involved in the anthocyanin biosynthetic pathway, including the structural genes: CHS, CHI, F3H, DFR1, DFR2 and ANS, known as MBW complex genes: MYB (M.alba_G0017209), MYB (M.alba_G0017689), bHLH (M.alba_G0012659), bHLH (M.alba_G0009347) and bHLH3 (M.alba_G0016257) and the ethylene response factor: ERF (M.alba_G0016603). Of these, changing trends related to expression pattern and anthocyanin content showed their most positive correlation at the post-flowering stage in both genotypes. Our results indicated that ethylene enhances anthocyanin accumulation in mulberry fruits. Furthermore, qRT-PCR was performed to confirm the above-mentioned genes' expression (except for MYB (M.alba_G0017689) and bHLH (M.alba_G0009347) was significantly up-regulated under ethylene treatment at 300 mg/L. These findings help uncover the gene regulatory networks of the anthocyanin biosynthetic pathway and will contribute to engineering purposes in future mulberry breeding programs.

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