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Genomic Characterisation of an Isolate of Brassica Yellows Virus Associated with Brassica Weed in Tasmania

文献类型： 外文期刊

作者： Umar, Muhammad 1 ; Farooq, Tahir 2 ; Tegg, Robert S. 1 ; Thangavel, Tamilarasan 1 ; Wilson, Calum R. 1 ;

作者机构： 1.Univ Tasmania, Tasmanian Inst Agr, New Town Res Labs, 13 St Johns Ave, Newtown, Tas 7008, Australia

2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

3.Bundaberg Res Facil, Dept Agr & Fisheries Queensland, 49 Ashfield Rd, Bundaberg, Qld 4670, Australia

关键词： Brassica yellows virus; genome characterisation; recombination; Polerovirus; phylogenetic analysis

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 7 期

页码：

收录情况： SCI

摘要： Brassica yellows virus (BrYV), a tentative species in the genus Polerovirus, of the Solemoviridae family, is a phloem-restricted and aphid-transmitted virus with at least three genotypes (A, B, and C). It has been found across mainland China, South Korea, and Japan. BrYV was previously undescribed in Tasmania, and its genetic variability in the state remains unknown. Here, we describe a nearcomplete genome sequence of BrYV (genotype A) isolated from Raphanus raphanistrum in Tasmania using next-generation sequencing and sanger sequencing of RT-PCR products. BrYV-Tas (GenBank Accession no. OM469309) possesses a genome of 5516 nucleotides (nt) and shares higher sequence identity (about 90%) with other BrYV isolates. Phylogenetic analyses showed variability in the clustering patterns of the individual genes of BrYV-Tas. Recombination analysis revealed beginning and ending breakpoints at nucleotide positions 1922 to 5234 nt, with the BrYV isolate LC428359 and BrYV isolate KY310572 identified as major and minor parents, respectively. Results of the evolutionary analysis showed that the majority of the codons for each gene are evolving under purifying selection, though a few codons were also detected to have positive selection pressure. Taken together, our findings will facilitate an understanding of the evolutionary dynamics and genetic diversity of BrYV.

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