您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Porcine circovirus type 2 ORF5 induces an inflammatory response by up-regulating miR-21 levels through targeting nuclear ssc-miR-30d

文献类型： 外文期刊

作者： Li, Chang 1 ; Yang, Keli 1 ; Song, Haofei 1 ; Xia, Chuqiao 1 ; Wu, Qiong 1 ; Zhu, Jiajia 1 ; Liu, Wei 1 ; Gao, Ting 1 ; Guo, Rui 1 ; Liu, Zewen 1 ; Yuan, Fangyan 1 ; Tian, Yongxiang 1 ; Zhou, Danna 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Hubei Prov Key Lab Anim Pathogen Microbiol,Minist, Wuhan 430064, Peoples R China

关键词： Porcine circovirus type 2; ORF5; Nuclear ssc-miR-30d; Ssc-miR-21; Inflammatory response

期刊名称：VIRUS RESEARCH （ 影响因子：2.5； 五年影响因子：3.2 ）

ISSN： 0168-1702

年卷期： 2024 年 346 卷

页码：

收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) infection leads to multi -system inflammation in pigs, and this effect can be achieved by upregulating host miR-21. The underlying mechanism of miR-21 regulates PCV2-induced inflammation is already known, however, how PCV2 regulates miR-21 levels and function using both autonomic and host factors remains to be further revealed. Here we present the first evidence that PCV2 ORF5 induces an inflammatory response by up-regulating miR-21 level through targeting nuclear miR-30d. In this study, we found that overexpression of ORF5 significantly increased miR-21 level and promoted the expression of inflammatory cytokines and activation of the NF- kappa B pathway, while ORF5 mutation had the opposite effect. Moreover, the differential expression of miR-21 could significantly change the pro-inflammatory effect of ORF5, indicating that ORF5 promotes inflammatory response by up-regulating miR-21. Bioinformatics analysis and clinical detection found that nuclear miR-30d was significantly down-regulated after ORF5 overexpression and PCV2 infection, and targeted pri-miR-21 and PCV2 ORF5. Functionally, we found that miR-30d inhibited the levels of miR-21 and inflammatory cytokines in cells. Mechanistically, we demonstrated that ORF5 inhibits miR-30d expression levels through direct binding but not via the circRNA pathway, and miR-30d inhibits miR-21 levels by targeting primiR-21. In summary, the present study revealed the molecular mechanism of ORF5 upregulation of miR-21, further refined the molecular chain of PCV2-induced inflammatory response and elucidated the role of miRNAs in it.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台