A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination
文献类型： 外文期刊
作者： Yang, Guangxin 1 ; Wei, Chunhao 1 ; Tang, Yunyu 1 ; Zhang, Jingru 1 ; Zhang, Xuan 1 ; Chi, Hai 1 ; Tao, Leren 3 ; Kong, Cong 1 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Minist Agr, Key Lab Control Qual & Safety Aquat Prod, Beijing 100000, Peoples R China
3.Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China
4.Univ Shanghai Sci & Technol, Sch Environm & Architecture, Shanghai 200093, Peoples R China
关键词： Vibrio parahaemolyticus; Gold nanoclusters; Fluorescence immunoassay; Inner Filter Effect
期刊名称：RESULTS IN CHEMISTRY （ 影响因子：2.3； 五年影响因子：2.3 ）
ISSN： 2211-7156
年卷期： 2021 年 3 卷
页码：
收录情况： SCI
摘要： A novel and sensitive fluorescence immunoassay was developed for the determination of Vibrio parahaemolyticus (VP) based on gold nanoclusters (BSA-AuNCs). The assay signal was originated from inner filter effect (IFE) between BSA-AuNCs and p-nitrophenol (PNP). A double emission peak fluorescent gold nanocluster with good property was quickly synthesized, which was used as fluorescent probe in this method. To achieve the IFE, Alkaline phosphatase labeled second antibody (IgG-ALP), which could generate PNP with the substrate of PNPP (p-nitrophenyl phosphate), was used in the immune reaction. According the competitive reaction, VP could be detected indirectly by the change of fluorescence signal. Under the optimal conditions, the limit of detection (LOD) for VP was 5 x 10(2) CFU/mL. The linear detection range was 10(3)-10(7) CFU/mL. Furthermore, the proposed method was successfully applied to detect VP in real samples, giving recoveries in the range of 82.5%-113.0%, and the coefficient of variation was 5.46%-9.88%.
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