Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses
文献类型： 外文期刊
作者： Xu, Qianru 1 ; Ma, Fanshu 2 ; Yang, Daichang 5 ; Li, Qingmei 3 ; Yan, Liming 7 ; Ou, Jiquan 6 ; Zhang, Longxian 2 ; Liu, Yunchao 3 ; Zhan, Quan 6 ; Li, Rui 3 ; Wei, Qiang 3 ; Hu, Hui 2 ; Wang, Yanan 3 ; Li, Xueyang 2 ; Zhang, Shenli 2 ; Yang, Jifei 3 ; Chai, Shujun 3 ; Du, Yongkun 2 ; Wang, Li 3 ; Zhang, Erqin 2 ; Zhang, Gaiping 2 ;
作者机构： 1.Henan Univ, Sch Basic Med Sci, Kaifeng, Peoples R China
2.Henan Agr Univ, Coll Vet Med, Int Joint Res Ctr Natl Anim Immunol, Zhengzhou, Peoples R China
3.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou, Peoples R China
4.Chinese Acad Sci, Suzhou Inst Nanotech & Nanob, CAS Key Lab Nanobio Interface, Suzhou, Peoples R China
5.Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China
6.Wuhan Healthgen Biotechnol Corp, Wuhan, Peoples R China
7.Tsinghua Univ, Sch Med, Lab Struct Biol, Beijing, Peoples R China
8.Longhu Lab, Zhengzhou, Peoples R China
9.Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China
关键词： transgenic rice; rational vaccine design; herringbone-dimer; Pestivirus; E2 protein
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）
ISSN： 1467-7644
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and required high doses. The development of new vaccines is therefore essential. Vaccine efforts should focus on optimizing antigen presentation to enhance immune responses. Here, we describe a simple herringbone-dimer strategy for efficient vaccine design, using the classical swine fever virus E2 expressed in a rice endosperm as an example. The expression of rE2 protein was identified, with the rE2 antigen accumulating to 480 mg/kg. Immunological assays in mice, rabbits, and pigs showed high antigenicity of rE2. Two immunizations with 284 ng of the rE2 vaccine or one shot with 5.12 lg provided effective protection in pigs without interference from pre-existing antibodies. Crystal structure and small-angle X-ray scattering results confirmed the stable herringbone dimeric conformation, which had two fully exposed duplex receptor binding domains. Our results demonstrated that rice endosperm is a promising platform for precise vaccine design, and this strategy can be universally applied to other Flaviviridae virus vaccines.
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