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Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB

文献类型： 外文期刊

作者： Zhong, Zuchang 1 ; Li, Benqiang 1 ; Tao, Jie 1 ; Cheng, Jinghua 1 ; Shi, Ying 1 ; Tang, Pan 1 ; Jiao, Jiajie 1 ; Liu, Huili 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Med, Shanghai 201106, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

3.Shanghai Key Lab Agr Genet Breeding, Shanghai 201106, Peoples R China

4.Shanghai Engn Res Ctr Pig Breeding, Shanghai 201302, Peoples R China

关键词： porcine sapelovirus; 3AB; indirect ELISA; infected and vaccinated animals

期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 1467-3037

年卷期： 2024 年 46 卷 9 期

页码：

收录情况： SCI

摘要： Porcine sapelovirus (PSV) is a new pathogen that negatively impacts the pig industry in China. Affected pigs experience severe diarrhea and even death. Vaccination is used to control disease outbreaks, and sensitive diagnostic methods that can distinguish infected animals from vaccinated animals (DIVA) are essential for monitoring the effectiveness of disease control programs. Tests based on the detection of the nonstructural protein (NSP) 3AB are reliable indicators of viral replication in infected and vaccinated animals. In this study, the recombinant PSV 3AB protein was expressed by a prokaryotic expression system, and an indirect ELISA method was established. Serum samples from healthy animals, immunized animals, and infected animals were evaluated. The ELISA method identified 3AB with high sensitivity (99.78%) and specificity (100.0%), and no cross-reaction was observed with serum antibodies against porcine reproductive and respiratory syndrome virus (PRRSV), infection with classical swine fever virus (CSFV), pseudorabies virus (PRV), bovine viral diarrhea virus (BVDV), porcine epidemic diarrhea virus (PEDV), or foot-and-mouth disease virus (FMDV). The ELISA method described here can effectively distinguish infected and vaccinated animals and is an important inexpensive tool for monitoring serum and controlling PSV.

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