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Growth, Enzymatic, and Transcriptomic Analysis of xyr1 Deletion Reveals a Major Regulator of Plant Biomass-Degrading Enzymes in Trichoderma harzianum

文献类型： 外文期刊

作者： Wang, Lunji 1 ; Zhao, Yishen 1 ; Chen, Siqiao 2 ; Wen, Xian 1 ; Anjago, Wilfred Mabeche 2 ; Tian, Tianchi 2 ; Chen, Yajuan 2 ; Zhang, Jinfeng 2 ; Deng, Sheng 2 ; Jiu, Min 1 ; Fu, Pengxiao 5 ; Zhou, Dongmei 2 ; Druzhinina, Irina S. 6 ; Wei, Lihui 2 ; Daly, Paul 2 ;

作者机构： 1.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing 210014, Peoples R China

3.Nanjing Agr Univ, Fungal Genom Lab FungiG, Nanjing 210095, Peoples R China

4.China Univ Min & Technol, Key Lab Coal Proc & Efficient Utilizat, Xuzhou 221116, Peoples R China

5.Jiangsu Coastal Ecol Sci & Technol Dev Co Ltd, Nanjing 210036, Peoples R China

6.Royal Bot Gardens Kew, Dept Accelerated Taxon, London TW9 3AE, England

关键词： CAZymes; XYR1/XlnR/XLR-1; cellulose; transcriptional regulation

期刊名称：BIOMOLECULES （ 影响因子：5.5； 五年影响因子：5.8 ）

ISSN：

年卷期： 2024 年 14 卷 2 期

页码：

收录情况： SCI

摘要： The regulation of plant biomass degradation by fungi is critical to the carbon cycle, and applications in bioproducts and biocontrol. Trichoderma harzianum is an important plant biomass degrader, enzyme producer, and biocontrol agent, but few putative major transcriptional regulators have been deleted in this species. The T. harzianum ortholog of the transcriptional activator XYR1/XlnR/XLR-1 was deleted, and the mutant strains were analyzed through growth profiling, enzymatic activities, and transcriptomics on cellulose. From plate cultures, the Delta xyr1 mutant had reduced growth on D-xylose, xylan, and cellulose, and from shake-flask cultures with cellulose, the Delta xyr1 mutant had similar to 90% lower beta-glucosidase activity, and no detectable beta-xylosidase or cellulase activity. The comparison of the transcriptomes from 18 h shake-flask cultures on D-fructose, without a carbon source, and cellulose, showed major effects of XYR1 deletion whereby the Delta xyr1 mutant on cellulose was transcriptionally most similar to the cultures without a carbon source. The cellulose induced 43 plant biomass-degrading CAZymes including xylanases as well as cellulases, and most of these had massively lower expression in the Delta xyr1 mutant. The expression of a subset of carbon catabolic enzymes, other transcription factors, and sugar transporters was also lower in the Delta xyr1 mutant on cellulose. In summary, T. harzianum XYR1 is the master regulator of cellulases and xylanases, as well as regulating carbon catabolic enzymes.

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