Discovery of the dipicolinic acid synthase in the spoilage fungus Paecilomyces reveals its role in spore heat, salt, and alkaline pH resistance
文献类型： 外文期刊
作者： Wang, Su-Yan 1 ; Zhang, Yun-Jiao 1 ; Chen, Li-Xiao 1 ; Pang, Yi-Bo 1 ; Herrera-Balandrano, Daniela D. 1 ; Daly, Paul 2 ; Liu, Feng-Quan 3 ; Simoes, Marta Filipa 4 ; Shi, Xin-Chi 1 ; Laborda, Pedro 1 ;
作者机构： 1.Nantong Univ, Sch Life Sci, Bldg 18,Off F604, Nantong 226019, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base Minist Sci & Technol, Nanjing, Peoples R China
3.Guizhou Univ, Coll Agr, Dept Plant Pathol, Guiyang, Peoples R China
4.Macau Univ Sci & Technol, State Key Lab Lunar & Planetary Sci, Taipa, Macao, Peoples R China
5.China Natl Space Adm CNSA, Macau Ctr Space Explorat & Sci, Taipa, Macao, Peoples R China
关键词： Food microbiology; Fungal spores; Environmental resistance; Structural biology; Food spoilage
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）
ISSN： 0963-9969
年卷期： 2025 年 205 卷
页码：
收录情况： SCI
摘要： Paecilomyces, a food spoilage fungus, releases airborne spores that are highly resistant to harsh environmental conditions, including pasteurization. In this study, dipicolinic acid (DPA), which is a common component of bacterial spores, was detected in Paecilomyces maximus conidia at concentrations from 0.1 % to 2.8 % w/w (DPA weight per spore weight). The DPA synthase (PmDpa) was identified and obtained through heterologous expression in Escherichia coli. The enzyme was iron-dependent and catalyzed the conversion of L-2,3-dihydrodipicolinate (DHD) into DPA in the presence of NAD+. Mutant Delta pmdpa spores exhibited altered morphology and reduced resistance to heat, salinity, and alkaline pH compared to wild-type spores, indicating that PmDpa plays a key role in the structure and environmental tolerance of P. maximus spores. DPA was identified in the spores of several Paecilomyces, Aspergillus, and Penicillium strains, indicating its prevalence in the conidia of Thermoascaceae and Aspergillaceae (Eurotiales). This study identifies DPA in Eurotiales spores for the first time and highlights the first key gene associated with Paecilomyces spore resistance.
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