文献类型： 外文期刊
作者： Guo, Lijin 1 ; Huang, Weiling 1 ; Wen, Qi 1 ; Zhang, Siyu 1 ; Bordbar, Farhad 1 ; Xiao, Zhengzhong 5 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Lingnan Guangdong Lab Agr, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
3.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
4.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
5.Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512005, Peoples R China
6.Queen Mary Univ London, Sch Biol & Behav Sci, London, England
关键词： G4s landscape; Embryonic period; Myogenesis; DNA methylation
期刊名称：BMC BIOLOGY （ 影响因子：4.5； 五年影响因子：5.4 ）
ISSN：
年卷期： 2024 年 22 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundDNA G-quadruplexes (G4s) represent a distinctive class of non-canonical DNA secondary structures. Despite their recognition as potential therapeutic targets in some cancers, the developmental role of G4 structures remains enigmatic. Mammalian embryonic myogenesis studies are hindered by limitations, prompting the use of chicken embryo-derived myoblasts as a model to explore G4 dynamics. This study aims to reveal the embryonic G4s landscape and elucidate the underlying mechanisms for candidate G4s that influence embryonic myogenesis.ResultsThis investigation unveils a significant reduction in G4s abundance during myogenesis. G4s stabilizer pyridostatin impedes embryonic myogenesis, emphasizing the regulatory role of G4s in this process. G4 Cut&Tag sequencing and RNA-seq analyses identify potential G4s and DEGs influencing embryonic myogenesis. Integration of G4 and DEG candidates identifies 32 G4s located in promoter regions capable of modulating gene transcription. WGBS elucidates DNA methylation dynamics during embryonic myogenesis. Coordinating transcriptome data with DNA G4s and DNA methylation profiles constructs a G4-DMR-DEG network, revealing nine interaction pairs. Notably, the NFATC2 promoter region sequence is confirmed to form a G4 structure, reducing promoter mCpG content and upregulating NFATC2 transcriptional activity.ConclusionsThis comprehensive study unravels the first embryonic genomic G4s landscape, highlighting the regulatory role of NFATC2 G4 in orchestrating transcriptional activity through promoter DNA methylation during myogenesis.
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