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High expression of miR-22-3p in chicken hierarchical follicles promotes granulosa cell proliferation, steroidogenesis, and lipid metabolism via PTEN/PI3K/Akt/mTOR signaling pathway

文献类型： 外文期刊

作者： Deng, Xun 1 ; Ning, Zifan 1 ; Li, Liang 3 ; Cui, Zhifu 5 ; Du, Xiaxia 1 ; Amevor, Felix Kwame 1 ; Tian, Yaofu 1 ; Shu, Gang 6 ; Du, Xiaohui 1 ; Han, Xue 3 ; Zhao, Xiaoling 1 ;

作者机构： 1.Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu, Peoples R China

2.Sichuan Agr Univ, Inst Anim Genet & Breeding, Key Lab Livestock & Poultry Multiomics, Minist Agr & Rural Affairs,Coll Anim Sci & Technol, Chengdu, Peoples R China

3.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang, Peoples R China

4.Guizhou Hongyu Anim Husb Technol Dev Co Ltd, Guiyang, Peoples R China

5.Southwest Univ, Coll Anim Sci & Technol, Chongqing, Peoples R China

6.Sichuan Agr Univ, Dept Basic Vet Med, Chengdu, Sichuan, Peoples R China

7.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang 550000, Peoples R China

8.Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu 611130, Sichuan, Peoples R China

关键词： miR-22-3p; Chicken granulosa cell; Proliferation; Steroid hormone; Lipid metabolism; PI3K/AKT/mTOR pathway

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 253 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are a class of RNA macromolecules that play regulatory roles in follicle development by inhibiting protein translation through binding to the 3'UTR of its target genes. Granulosa cell (GC) proliferation, steroidogenesis, and lipid metabolism have indispensable effect during folliculogenesis. In this study, we found that miR-22-3p was highly expressed in the hierarchical follicles of the chickens, which indicated that it may be involved in follicle development. The results obtained suggested that miR-22-3p promoted proliferation, hormone secretion (progesterone and estrogen), and the content of lipid droplets (LDs) in the chicken primary GC. The results from the bioinformatics analysis, luciferase reporter assay, qRT-PCR, and Western blotting, confirmed that PTEN was directly targeted to miR-22-3p. Subsequently, it was revealed that PTEN inhibited proliferation, hormone secretion, and the content of LDs in GC. Therefore, this study showed that miR-22-3p could activate PI3K/Akt/mTOR pathway via targeting PTEN. Taken together, the findings from this study indicated that miR22-3p was highly expressed in the hierarchical follicles of chickens, which promotes GC proliferation, steroidogenesis, and lipid metabolism by repressing PTEN to activate PI3K/AKT/mTOR pathway.

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