文献类型： 外文期刊

作者： Xu, Yuhui ¹ ; Li, Haoxia ² ; Shi, Tongwei ³ ; Luo, Qing ¹ ; Chen, Yuchao ⁴ ; Guo, Shenghu ⁴ ; Tian, Weiwei ⁵ ; An, Wei ¹ ; Zhao, Jian ¹ ; Yin, Yue ¹ ; He, Jun ¹ ; Zheng, Rui ⁶ ; Liang, Xiaojie ¹ ; Wang, Yajun ¹ ; Zhang, Xiyan ¹ ; Shi, Zhigang ¹ ; Duan, Linyuan ¹ ; Qin, Xiaoya ¹ ; Huang, Ting ¹ ; Zhang, Bo ¹ ; Wan, Ru ¹ ; Li, Yanlong ¹ ; Cao, Youlong ¹ ; Liu, Hui ⁸ ; Shu, Sheng ⁸ ; Xiong, Aisheng ⁸ ; Zhao, Jianhua ¹ ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Wolfberry Sci Res Inst, Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Forestry & Grassland Ecol, Yinchuan 750002, Peoples R China

3.Planttech Technol Co Ltd, Beijing, Peoples R China

4.Ningxia Acad Agr & Forestry Sci, Agr Biotechnol Ctr, Yinchuan 750002, Peoples R China

5.Sichuan Acad Chinese Med Sci, Chengdu 610041, Peoples R China

6.Ningxia Univ, Key Lab Minist Educ Protect & Utilizat Special Bio, Yinchuan 750021, Peoples R China

7.Ningxia Univ, Coll Life Sci, Yinchuan 750021, Peoples R China

8.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement &, Minist Agr & Rural Affairs,Key Lab Biol & Germplas, Nanjing 210095, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）

ISSN： 2662-6810

年卷期： 2025 年 12 卷 2 期

页码：

收录情况： SCI

摘要： Black wolfberry (Lycium ruthenicum Murr.) is an important plant for ecological preservation. In addition, its fruits are rich in anthocyanins and have important edible and medicinal value. However, a high-quality chromosome-level genome for this species is not yet available, and the regulatory mechanisms involved in the biosynthesis of anthocyanins are unclear. In this study, haploid material was used to assemble a high-quality chromosome-level reference genome of Lycium ruthenicum, resulting in a genome size of 2272 Mb with contig N50 of 92.64 Mb, and 38 993 annotated gene models. In addition, the evolution of this genome and large-scale variations compared with the Ningxia wolfberry Lycium barbarum were determined. Importantly, homology annotation identified 86 genes involved in the regulatory pathway of anthocyanin biosynthesis, five of which [LrCHS1 (evm.TU.Chr05.295), LrCHS2 (evm.TU.Chr09.488), LrAOMT (evm.TU.Chr09.809), LrF3'5'H (evm.TU.Chr06.177), and LrAN2.1 (evm.TU.Chr05.2618)] were screened by differential expression analysis and correlation analysis using a combination of transcriptome and metabolome testing. Overexpression of these genes could significantly up- or downregulate anthocyanin-related metabolites. These results will help accelerate the functional genomic research of L. ruthenicum, and the elucidation of the genes involved in anthocyanin synthesis will be beneficial for breeding new varieties and further exploring its ecological conservation potential.