The LbNAM2-LbZDS module enhances drought resistance in wolfberry (Lycium barbarum) by participating in ABA biosynthesis
文献类型： 外文期刊
作者： Ma, Fang 1 ; Liang, Yunfei 1 ; Meng, Fanyi 1 ; Yang, Peizhi 3 ; Guo, Cong 1 ; Shi, Hongyan 1 ; Ma, Mengqiu 1 ; Wang, Yuqin 4 ; Feng, Ru 5 ; Cai, Yiyong 6 ; Hu, Tixu 1 ; Chen, Rugang 1 ; Yin, Yue 7 ; Zhan, Xiangqiang 1 ;
作者机构： 1.Northwest A&F Univ, State Key Lab Crop Stress Resistance & High Effici, Yangling 712100, Peoples R China
2.Northwest A&F Univ, Coll Hort, Yangling 712100, Peoples R China
3.Northwest A&F Univ, Coll Grassland Agr, Yangling 712100, Peoples R China
4.Anhui Agr Univ, Sch Life Sci, Hefei 230036, Anhui, Peoples R China
5.Ulanqab Agr Technol & Popularizat Ctr, Orthoped Ctr, Ulanqab 012000, Inner Mongolia, Peoples R China
6.Shaanxi Jinpeng Seed Ind Co Ltd, Yangling 712100, Peoples R China
7.Ningxia Acad Agr & Forestry Sci, Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China
关键词： wolfberry; LbZDS; drought stress; carotenoids; LbNAM2; ABA biosynthesis
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2025 年 121 卷 6 期
页码：
收录情况： SCI
摘要： Wolfberry (Lycium barbarum L.) fruit, renowned for its high carotenoid content, is extensively used in traditional Chinese herbal medicine and cuisine. Drought is a significant global challenge to crop production, with carotenoids playing crucial roles in enhancing drought resistance in higher plants. zeta-Carotene desaturase (ZDS), a key enzyme in the carotenoid biosynthesis pathway, catalyzes the conversion of zeta-carotene to lycopene. However, the molecular mechanisms by which LbZDS responds to drought stress remain largely unexplored. In this study, we demonstrated that LbZDS transcription is induced by PEG, NaCl, and abscisic acid (ABA) treatments. Overexpression of LbZDS in both wolfberry and tomato plants conferred enhanced drought tolerance by promoting ABA synthesis. We further identified that the NAC transcription factor LbNAM2 directly binds to the promoter region of LbZDS and activates its expression, as evidenced by electrophoretic mobility shift assays, yeast one-hybrid assays, and dual-luciferase assays. Silencing LbNAM2, or dual silencing of LbNAM2 and LbZDS via virus-induced gene silencing (VIGS), severely compromised drought tolerance in wolfberry plants. Additionally, overexpression of LbZDS resulted in a marked increase in carotenoid content, while silencing either LbZDS, LbNAM2, or both together led to reduced carotenoid levels. In conclusion, our study provides critical insights into the functional roles and regulatory mechanisms of the LbNAM2-LbZDS module in drought stress response and carotenoid biosynthesis in wolfberry.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification of bZIP Gene Family in Lycium barbarum and Expression During Fruit Development
作者：Gao, Han;Zheng, Rui;Cao, Xiaoyu;Qin, Xiaoya;Bai, Xiaorong;Zhang, Xiyan;Yin, Yue;Ma, Yunni;Xiong, Aisheng;Zheng, Rui
关键词：
Lycium barbarum ;bZIP gene family; evolutionary analysis; gene expression; subcellular localization -
High-quality genome of black wolfberry (Lycium ruthenicum Murr.) provides insights into the genetics of anthocyanin biosynthesis regulation
作者：Xu, Yuhui;Luo, Qing;An, Wei;Zhao, Jian;Yin, Yue;He, Jun;Liang, Xiaojie;Wang, Yajun;Zhang, Xiyan;Shi, Zhigang;Duan, Linyuan;Qin, Xiaoya;Huang, Ting;Zhang, Bo;Wan, Ru;Li, Yanlong;Cao, Youlong;Zhao, Jianhua;Li, Haoxia;Shi, Tongwei;Chen, Yuchao;Guo, Shenghu;Tian, Weiwei;Zheng, Rui;Zheng, Rui;Liu, Hui;Shu, Sheng;Xiong, Aisheng
关键词：
-
Wolfberry genome database: integrated genomic datasets for studying molecular biology
作者：Cao, You-Long;Li, Yan-Long;Fan, Yun-Fang;Luo, Qing;Zhao, Jian-Hua;Yin, Yue;An, Wei;Shi, Zhi-Gang;Cao, You-Long;Li, Yan-Long;Fan, Yun-Fang;Luo, Qing;Zhao, Jian-Hua;Yin, Yue;An, Wei;Shi, Zhi-Gang;Chen, You-Yi;Li, Chung-, I;Lin, Shao-Ting;Tsai, Wen-Chieh;Lin, Shao-Ting;Tsai, Wen-Chieh;Lee, Bing-Ru;Hsieh, Chun-Lin;Wu, Wei-Sheng;Hsiao, Yu-Yun;Tsai, Wen-Chieh;Chow, Chi-Nga;Chang, Wen-Chi;Tsai, Wen-Chieh;Huang, Chun-Lin;Chang, Wei-Hung;Chang, Wei-Hung;Liu, Zhong-Jian;Liu, Zhong-Jian;Liu, Zhong-Jian;Liu, Zhong-Jian;Tsai, Wen-Chieh;Tsai, Wen-Chieh
关键词：wolfberry; Lycium barbarum; wolfberry genome database (WGDB); user-friendly; genomics; transcriptomics
-
Metabolite-based genome-wide association studies enable the dissection of the genetic bases of flavonoids, betaine and spermidine in wolfberry (Lycium)
作者：Zhao, Jianhua;An, Wei;Yin, Yue;Duan, Linyuan;Ren, Xiaoyue;Liang, Xiaojie;Wang, Yajun;Wan, Ru;Huang, Ting;Zhang, Bo;Li, Yanlong;Cao, Youlong;Xu, Yuhui;Li, Haoxia;Wang, Bin;Wang, Liping;Wang, Bi;Luo, Jie
关键词：Wolfberry; genome assembly; metabolite-based genome-wide association study; bioactivity metabolites; candidate gene
-
Anthocyanin biosynthesis in goji berry is inactivated by deletion in a bHLH transcription factor LrLAN1b promoter
作者：Li, Tingting;Wang, Jingjin;Zhang, Zihan;Qin, Huan;Tang, Lin;Fan, Yunfang;Yin, Yue;Dai, Guoli;Cao, Youlong;Fan, Yunfang;Yin, Yue;Dai, Guoli;Cao, Youlong
关键词：
-
Comparative Analysis of the GATA Transcription Factors in Five Solanaceae Species and Their Responses to Salt Stress in Wolfberry (Lycium barbarum L.)
作者：Zhang, Fengfeng;Wu, Yan;Shi, Xin;Wang, Xiaojing;Yin, Yue
关键词：wolfberry; GATA gene family; Solanaceae; salt stress; gene expression
-
ERF5.1 modulates carotenoid accumulation by interacting with CCD4.1 in Lycium
作者：Zhao, Jianhua;Xu, Yuhui;Zhu, Xinlei;Yin, Yue;Zhang, Xiyan;Duan, Linyuan;Liang, Xiaojie;Huang, Ting;Zhang, Bo;Wan, Ru;Shi, Zhigang;Cao, Youlong;An, Wei;Li, Haoxia;Zhou, Jun
关键词：
