Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding opaque2, opaque16 and waxy1 genes in maize
文献类型： 外文期刊
作者： Wu, Peizhen 2 ; Yuan, Yanli 1 ; Ma, Zhoujie 2 ; Zhang, Kaiwu 2 ; Deng, Lei 2 ; Ren, Hong 2 ; Yang, Wenpeng 2 ; Wang, Wei 2 ;
作者机构： 1.Guizhou Univ, Inst Agrobioengineering, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang 550025, Guizhou, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Inst Upland Food Crops, Guiyang 550006, Guizhou, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Maize Biol & Genet Breeding Karst Mt, Guiyang 550006, Peoples R China
关键词： lysine; tryptophan; o2; o16; wx; gene pyramiding; differentially expressed genes
期刊名称：BREEDING SCIENCE （ 影响因子：2.0； 五年影响因子：2.4 ）
ISSN： 1344-7610
年卷期： 2024 年
页码：
收录情况： SCI
摘要： To explore the molecular mechanism behind maize grain quality and use of different gene stacking to im- prove the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing o2o16wx, along with the double-recessive mutant lines containing o2o16, o2wx, and o16wx. The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (o2o16wx, o2wx, and o16wx) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis. In the lysine degradation pathway, 'QCL8010_1' (o2o16wx) revealed that the gene encoding saccharopine dehydrogenase (LKR/SDH) was down-regulated. In addition, the gene pyramid- ing lines (o2o16wx, o2o16, and o16wx) indicated that the gene encoding 2-oxoglutarate dehydrogenase E1 component (OGDH) was down-regulated, inhibiting the degradation of lysine. In the tryptophan synthesis pathway, the genes encoding anthranilate synthase (AS), anthranilate synthase (APT), and tryptophan syn- thase (TS) were up-regulated (in o2o16wx, o2o16, o2wx, and o16wx), and promote tryptophan synthesis. In the tryptophan degradation pathway, it was revealed that the genes encoding indole-3-producing oxidase (IAAO) and indole-3-pyruvate monooxygenase (YUCCA) were down-regulated. These results provide a ref- erence for revealing the mechanism of the o2, o16, and wx with different gene pyramiding to improve grain quality in maize.
- 相关文献
作者其他论文 更多>>
-
RNA-Seq Revealed the Molecular Mechanism of Nutritional Quality Improvement in o16-ωx Double-Mutation Maize
作者：Ma, Zhoujie;Wu, Peizhen;Song, Li;Deng, Lei;Zhang, Kaiwu;Yang, Wenpeng;Ren, Hong;Wang, Wei;Deng, Lei;Zhang, Kaiwu;Yang, Wenpeng;Ren, Hong;Wang, Wei
关键词：opaque16; lysine; tryptophan; RNA-seq; Zea mays L.
-
DNA Barcoding of Fish Species Diversity in Guizhou, China
作者：Tang, Qian;Deng, Lei;Luo, Qi;Duan, Qian;Zhang, Renyi;Wang, Xue
关键词：fish; DNA barcode; species delimitation; Guizhou province
-
Estimation of maize straw production and appropriate straw return rate in China
作者：Liu, Wanmao;Liu, Guangzhou;Xie, Ruizhi;Ming, Bo;Wang, Keru;Xue, Jun;Li, Shaokun;Hou, Peng;Liu, Wanmao;Liu, Yuee;Yang, Yunshan;Guo, Xiaoxia;Wang, Yonghong;Zhao, Rulang;Zhang, Wenjie;Wang, Yongjun;Bian, Shaofeng;Ren, Hong;Zhao, Xiaoyan;Liu, Peng;Chang, Jianzhi;Zhang, Guohe;Liu, Jiayou;Yuan, Liuzheng;Zhao, Haiyan;Shi, Lei;Zhang, Lili;Yu, Lin;Gao, Julin;Yu, Xiaofang;Wang, Zhigang;Shen, Liguo;Ji, Ping;Yang, Shuzong;Zhang, Zhongdong;Xue, Jiquan;Wang, Xiuquan;Wang, Xiuquan;Lu, Tingqi;Dong, Benchun;Li, Gang;Ma, Baoxin;Li, Jinqin;Deng, Xiufeng;Liu, Yonghong;Yang, Qin;Jia, Chunlan;Chen, Xianping;Fu, Hua
关键词：Maize straw production; Straw return; Carbon input; Carbon emission; Soil carbon sequestration
-
Estimation of maize straw production and appropriate straw return rate in China
作者：Liu, Wanmao;Liu, Guangzhou;Xie, Ruizhi;Ming, Bo;Wang, Keru;Xue, Jun;Li, Shaokun;Hou, Peng;Liu, Wanmao;Liu, Yuee;Yang, Yunshan;Guo, Xiaoxia;Wang, Yonghong;Zhao, Rulang;Zhang, Wenjie;Wang, Yongjun;Bian, Shaofeng;Ren, Hong;Zhao, Xiaoyan;Liu, Peng;Chang, Jianzhi;Zhang, Guohe;Liu, Jiayou;Yuan, Liuzheng;Zhao, Haiyan;Shi, Lei;Zhang, Lili;Yu, Lin;Gao, Julin;Yu, Xiaofang;Wang, Zhigang;Shen, Liguo;Ji, Ping;Yang, Shuzong;Zhang, Zhongdong;Xue, Jiquan;Wang, Xiuquan;Wang, Xiuquan;Lu, Tingqi;Dong, Benchun;Li, Gang;Ma, Baoxin;Li, Jinqin;Deng, Xiufeng;Liu, Yonghong;Yang, Qin;Jia, Chunlan;Chen, Xianping;Fu, Hua
关键词：Maize straw production; Straw return; Carbon input; Carbon emission; Soil carbon sequestration
-
Transcriptome Profiling of Different State Callus Induced from Immature Embryo in Maize
作者：Zhang, Kaiwu;Wang, Wei;Du, Dengxiang
关键词：
-
A Rapid Pipeline for Pollen- and Anther-Specific Gene Discovery Based on Transcriptome Profiling Analysis of Maize Tissues
作者：Shi, Yannan;Li, Yao;Guo, Yongchao;Ma, Zhengqiang;Yan, Yuanxin;Borrego, Eli James;Wei, Zhengyi;Ren, Hong;Ma, Zhengqiang;Yan, Yuanxin
关键词：Seed Production Technology; crop breeding; hybrids; male-sterile; maize
-
Quantifying maize grain yield losses caused by climate change based on extensive field data across China
作者：Hou, Peng;Liu, Wanmao;Xie, Ruizhi;Wang, Keru;Ming, Bo;Liu, Guangzhou;Xue, Jun;Li, Shaokun;Liu, Yuee;Liu, Wanmao;Yang, Haishun;Wang, Yonghong;Zhao, Rulang;Zhang, Wenjie;Wang, Yongjun;Bian, Shaofeng;Ren, Hong;Zhao, Xiaoyan;Liu, Peng;Chang, Jianzhi;Zhang, Guohe;Liu, Jiayou;Yuan, Liuzheng;Zhao, Haiyan;Shi, Lei;Zhang, Lili;Yu, Lin;Gao, Julin;Yu, Xiaofang;Wang, Zhigang;Shen, Liguo;Ji, Ping;Yang, Shuzong;Zhang, Zhongdong;Xue, Jiquan;Ma, Xiangfeng;Wang, Xiuquan;Lu, Tingqi;Dong, Benchun;Li, Gang;Ma, Baoxin;Li, Jinqin;Deng, Xiufeng;Liu, Yonghong;Yang, Qin;Jia, Chunlan;Chen, Xianping;Fu, Hua
关键词：Maize; Grain yield; Climate change; Temperature; Solar radiation
