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Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding opaque2, opaque16 and waxy1 genes in maize

文献类型： 外文期刊

作者： Wu, Peizhen 2 ; Yuan, Yanli 1 ; Ma, Zhoujie 2 ; Zhang, Kaiwu 2 ; Deng, Lei 2 ; Ren, Hong 2 ; Yang, Wenpeng 2 ; Wang, Wei 2 ;

作者机构： 1.Guizhou Univ, Inst Agrobioengineering, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang 550025, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Inst Upland Food Crops, Guiyang 550006, Guizhou, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Maize Biol & Genet Breeding Karst Mt, Guiyang 550006, Peoples R China

关键词： lysine; tryptophan; o2; o16; wx; gene pyramiding; differentially expressed genes

期刊名称：BREEDING SCIENCE （ 影响因子：2.0； 五年影响因子：2.4 ）

ISSN： 1344-7610

年卷期： 2024 年

页码：

收录情况： SCI

摘要： To explore the molecular mechanism behind maize grain quality and use of different gene stacking to im- prove the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing o2o16wx, along with the double-recessive mutant lines containing o2o16, o2wx, and o16wx. The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (o2o16wx, o2wx, and o16wx) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis. In the lysine degradation pathway, 'QCL8010_1' (o2o16wx) revealed that the gene encoding saccharopine dehydrogenase (LKR/SDH) was down-regulated. In addition, the gene pyramid- ing lines (o2o16wx, o2o16, and o16wx) indicated that the gene encoding 2-oxoglutarate dehydrogenase E1 component (OGDH) was down-regulated, inhibiting the degradation of lysine. In the tryptophan synthesis pathway, the genes encoding anthranilate synthase (AS), anthranilate synthase (APT), and tryptophan syn- thase (TS) were up-regulated (in o2o16wx, o2o16, o2wx, and o16wx), and promote tryptophan synthesis. In the tryptophan degradation pathway, it was revealed that the genes encoding indole-3-producing oxidase (IAAO) and indole-3-pyruvate monooxygenase (YUCCA) were down-regulated. These results provide a ref- erence for revealing the mechanism of the o2, o16, and wx with different gene pyramiding to improve grain quality in maize.

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