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Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L

文献类型： 外文期刊

作者： Xiong, Xingpeng 1 ; Li, Xiaoyao 2 ; Zhang, Shuting 2 ; Hu, Zhen 2 ; Liu, Tingting 3 ; Qiu, Zhengming 2 ; Cao, Jiashu 4 ; Huang, Li 4 ; Yan, Chenghuan 2 ;

作者机构： 1.Jingchu Univ Technol, Coll Bioengn, Jingmen 448000, Peoples R China

2.Hubei Acad Agr Sci, Inst Econ Crops, Key Lab Vegetable Ecol Cultivat Highland, Minist Agr & Rural Affairs,Hubei Key Lab Vegetable, Wuhan, Hubei, Peoples R China

3.Zhejiang Inst Landscape Plants & Flowers, Hangzhou 311251, Peoples R China

4.Zhejiang Univ, Inst Vegetable Sci, Lab Cell & Mol Biol, Hangzhou 310058, Peoples R China

关键词： Brassica rapa; Brmmd1; Fine mapping; Genic male sterility

期刊名称：GENE （ 影响因子：2.6； 五年影响因子：2.7 ）

ISSN： 0378-1119

年卷期： 2024 年 924 卷

页码：

收录情况： SCI

摘要： Recessive genic male sterility (RGMS) provides an effective approach for the commercial exploitation of heterosis, especially in Brassica crops. Although some artificial RGMS mutants have been reported in B. rapa, no causal genes derived from these natural mutants have been identified so far. In this study, a spontaneous RGMS mutant Bcajh97-01A derived from the 'Aijiaohuang' line traced back to the 1980 s was identified. Genetic analysis revealed that the RGMS trait was controlled by a single locus in the Bcajh97-01A/B system. Bulk segregant analysis (BSA) in combination with linkage analysis was employed to delimit the causal gene to an approximate 129 kb interval on chromosome A02. The integrated information of transcriptional levels and the predicted genes in the target region indicated that the Brmmd1 (BraA02g017420) encoding a PHD-containing nuclear protein was the most likely candidate gene. A 374 bp miniature inverted-repeat transposable element (MITE) was inserted into the first exon to prematurely stop the Brmmd1 gene translation, thus blocking the normal expression of this gene at the tetrad stage in the Bcajh97-01A. Additionally, a co-segregating structure variation (SV) marker was developed to rapidly screen the RGMS progenies from Bcajh97-01A/B system. Our findings reveal that BraA02g017420 is the causal gene responsible for the RGMS trait. This study lays a foundation for marker-assisted selection and further molecular mechanism exploration of pollen development in B. rapa.

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