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Chemical composition analysis and transcriptomics reveal the R2R3- MYB genes and phenol oxidases regulating the melanin formation in black radish

文献类型： 外文期刊

作者： Zhang, Shuting 1 ; Yuan, Guoli 1 ; Peng, Zhaoxin 1 ; Li, Xiaoyao 3 ; Huang, Yan 1 ; Yin, Chaomin 4 ; Cui, Lei 1 ; Xiao, Guilin 1 ; Jiao, Zhenbiao 1 ; Wang, Liping 3 ; Deng, Xiaohui 1 ; Qiu, Zhengming 1 ; Yan, Chenghuan 1 ;

作者机构： 1.Hubei Acad Agr Sci, Ind Crops Inst, Key Lab Vegetable Ecol Cultivat Highland, Hubei Hongshan Lab,Minist Agr & Rural Affairs, Wuhan 430063, Hubei, Peoples R China

2.Hubei Key Lab Vegetable Germplasm Enhancement & Ge, Wuhan 430063, Hubei, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Hubei, Peoples R China

4.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China

关键词： Correlation network; Melanin formation; Phenol oxidases; MYB genes; Raphanus sativus

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）

ISSN： 0141-8130

年卷期： 2024 年 271 卷

页码：

收录情况： SCI

摘要： Melanins are dark-brown to black-colored biomacromolecules which have been thoroughly studied in animals and microorganisms. However, the biochemical and molecular basis of plant melanins are poorly understood. We first characterized melanin from the black radish ( Raphanus sativus var. niger ) 'HLB ' through spectroscopic techniques. p -Coumaric acid was identified as the main precursor of radish melanin. Moreover, a joint analysis of transcriptome and coexpression network was performed for the two radish accessions with black and white cortexes, 'HLB ' and '55 ' . A set of R2R3-type RsMYBs and enzyme-coding genes exhibited a coexpression pattern, and were strongly correlated with melanin formation in radish. Transient overexpression of two phenol oxidases RsLAC7 (laccase 7) or RsPOD22 - 1 (peroxidase 22-1) resulted in a deeper brown color around the infiltration sites and a significant increase in the total phenol content. Furthermore, co-injection of the transcriptional activator RsMYB48/RsMYB97 with RsLAC7 and/or RsPOD22 - 1 , markedly increased the yield of black extracts. Spectroscopic analyses revealed that these extracts are similar to the melanin found in 'HLB ' . Our findings advance the understanding of structural information and the transcriptional regulatory mechanism underlying melanin formation in radish.

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