Molecular characteristics and hypovirulence of different RNA viruses in Alternaria spp. strains, the pathogenic agent of pear black spot disease in China
文献类型： 外文期刊
作者： He, Ying 1 ; Wang, Qiong 1 ; Yang, Yuekun 1 ; Li, Yanshen 2 ; Zhu, Hongyan 3 ; Hong, Ni 1 ; Wang, Guoping 1 ; Wang, Liping 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China
2.Yantai Univ, Yantai 264005, Shandong Provin, Peoples R China
3.Hubei Acad Agr Sci, Res Inst Fruit & Tea, Hubei Key Lab Germplasm Innovat & Utilizat Pruit T, Wuhan 430070, Hubei Province, Peoples R China
4.Plant Pathol, Wuhan 430070, Hubei Province, Peoples R China
关键词： Pear black spot disease; Alternaria spp.; Mycovirus; High-throughput sequencing (HTS); Molecular diversity; Biological control
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）
ISSN： 0304-4238
年卷期： 2024 年 325 卷
页码：
收录情况： SCI
摘要： Pear black spot (Alternaria spp.) disease widespread in most pear production regions in China, causes early defoliation, which leads to the decline of fruit quality and heavily restricts the healthy development of the pear industry. Presently, no efficient way was found to biocontrol pear black spot disease. The mycovirus-mediated hypovirulent strain is regarded as an important measure of biocontrol of the fungal disease. Therefore, it is particularly urgent to excavate mycoviral resources with hypovirulence in Alternaria spp. strains. This study obtained partial or complete genomes of 14 mycoviruses from 83 Alternaria spp. strains from Wuhan city of China by high throughput sequencing, including 3 double-stranded (ds) RNA and 11 positive single-stranded (+ss) types, which were grouped into 8 distinct lineages, belonging to Chrysoviridae (isolate of AaCV1), Curvulaviridae (one virus), Partitiviridae (one virus), Mitoviridae (three viruses), Botourmiaviridae (five viruses), Deltaflexiviridae (one virus), Fusariviridae (one virus) and Togaviridae (one virus). The multiple mycoviral coinfections were common and prevalent, accounting for 78 % (65/83), with each strain harboring different numbers of mycoviruses, ranging from two to seven in Alternaria spp. Furthermore, the effects of the mycoviruses on the host were analyzed to screen the viruses-inducing hypovirulent strains. The derivative isolates from hypovirulent HB145 with different mycoviruses were obtained by vertical transmission. It revealed that the mycoviruses have different effects on the virulence of derivative isolates from HB-145. In conclusion, these findings provide important molecular information for the classification, origin, and genetic variety of mycoviruses, and candidate materials for the exploitation of mycoviruses as biocontrol agents.
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