TAGGING PATHOGENICITY GENES IN FUSARIUM GRAMINEARUM USING THE TRANSPOSON SYSTEM MIMP/IMPALA
文献类型: 外文期刊
作者: Dufresne, Marie 1 ; Van der Lee, Theo 2 ; Ben M'barek, Sarrah 1 ; Xu, X. 2 ; Zhang, X. 2 ; Liu, Taiguo 1 ; Zhang, Wenw 1 ;
作者机构: 1.Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France
2.Plant Res Int BV, NL-6500 AA Wageningen, Netherlands
3.Liaoning Acad Agr Sci, Inst Plant Protect, Shenyang 110161, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Genet, Nanjing 210014, Peoples R China
5.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China
期刊名称:CEREAL RESEARCH COMMUNICATIONS ( 影响因子:0.85; 五年影响因子:1.067 )
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收录情况: SCI
摘要: Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimpl/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) ofgenes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation witha non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.
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