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RNAi-mediated pest control targeting the Troponin I (wupA) gene in sweet potato weevil, Cylas formicarius

文献类型： 外文期刊

作者： Zhang, Mengjun 1 ; Zhang, Xiaxuan 1 ; Chen, Tingting 1 ; Liao, Yonglin 3 ; Yang, Bin 1 ; Wang, Guirong 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Minist Agr & Rural Affairs, Guangdong Lab Lingnan Modern Agr,Synthet Biol Lab, Shenzhen, Guangdong Provi, Peoples R China

3.Guangdong Acad Agr Sci, Inst Plant Protect, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou, Peoples R China

4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

关键词： biopesticides; gene knockdown; IPM; pest management; RNA interference; wupA

期刊名称：INSECT SCIENCE （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 1672-9609

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The sweet potato weevil (Cylas formicarius) is a critical pest producing enormous global losses in sweet potato crops. Traditional pest management approaches for sweet potato weevil, primarily using chemical pesticides, causes pollution, food safety issues, and harming natural enemies. While RNA interference (RNAi) is a promising environmentally friendly approach to pest control, its efficacy in controlling the sweet potato weevil has not been extensively studied. In this study, we selected a potential target for controlling C. formicarius, the Troponin I gene (wupA), which is essential for musculature composition and crucial for fundamental life activities. We determined that wupA is abundantly expressed throughout all developmental stages of the sweet potato weevil. We evaluated the efficiency of double-stranded RNAs in silencing the wupA gene via microinjection and oral feeding of sweet potato weevil larvae at different ages. Our findings demonstrate that both approaches significantly reduced the expression of wupA and produced high mortality. Moreover, the 1st instar larvae administered dswupA exhibited significant growth inhibition. We assessed the toxicity of dswupA on the no-target insect silkworm and assessed its safety. Our study indicates that wupA knockdown can inhibit the growth and development of C. formicarius and offer a potential target gene for environmentally friendly control.

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