Key residues of Bacillus thuringiensis Cry2Ab for oligomerization and pore-formation activity
文献类型： 外文期刊
作者： Pan, Zhi-Zhen 1 ; Xu, Lian 2 ; Liu, Bo 1 ; Chen, Qing-Xi 2 ; Zhu, Yu-Jing 1 ;
作者机构： 1.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Peoples R China
2.Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China
关键词： Cry2Ab; Oligomerization; Insecticidal activity; Pore-forming activity
期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）
ISSN： 2191-0855
年卷期： 2021 年 11 卷 1 期
页码：
收录情况： SCI
摘要： As a pore-forming toxin, activation, oligomerization and pore-formation were both required for the mode of action of Cry toxins. Previous results revealed that the helices alpha 4-alpha 5 of Domain I were involved in the oligomerization of Cry2Ab, however, the key residues for Cry2Ab aggregation remained ambiguous. In present studies, we built 20 Cry2Ab alanine mutants site-directed in the helices alpha 4-alpha 5 of Domain I and demonstrated that mutants N151A, T152A, F157A, L183A, L185A and I188A could reduce the assembly of the 250 kDa oligomers, suggesting that these mutation residues might be essential for Cry2Ab oligomerization. As expected, all of these variants showed lower insecticidal activity against P. xylostella. Furthermore, we found that the pore-forming activities of these mutants also decreased when compared to wild-type Cry2Ab. Taken together, our data identified key residues for Cry2Ab oligomerization and emphasized that oligomerization was closely related to the insecticidal activity and pore-forming activity of Cry2Ab.
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