您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The formation of pineapple translucency was regulated by Ca2+/H+ antiporter gene AcoCAX2

文献类型： 外文期刊

作者： Shu, Haiyan 1 ; Vafadar, Farinaz 3 ; Luan, Aiping 1 ; Wang, You 1 ; He, Junhu 1 ; Zhan, Rulin 1 ; Chang, Shenghe 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Hainan, Peoples R China

3.Isfahan Univ Technol, Coll Agr, Dept Agron & Plant Breeding, Esfahan 8415683111, Iran

4.Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Breeding, Sanya 572025, Peoples R China

关键词： Pineapple; Flesh translucency; Calcium deficiency; Stalk; Ca2+/H+ antiporter exchanger (CAX)

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

ISSN：

年卷期： 2025 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Background Flesh translucency is a significant physiological disorder in pineapple cultivation. Calcium deficiency in the fruit is a primary cause of flesh translucency. However, simply adding calcium to the soil or applying calcium fertilizer to the leaves and fruits does not eliminate flesh translucency. The mechanism behind this phenomenon has not been previously documented. Results Our research discovered that the calcium content in the stalk was significantly higher than in the fruit. Calcium ions are primarily transported through the phloem. The calcium concentration in the phloem sap of pineapple stalk at different stages ranged from 14 to 17 mu mol/L. The transporter responsible for unloading Ca2+ across the membrane of the end sieve vessel in the stalk phloem is the Ca2+/H+ antiporter exchanger (CAX). Among the four CAX genes in pineapple, only AcoCAX2 is expressed in the stalk. The AcoCAX2 protein is located in the plasma membrane and cytoplasm. Calcium-sensitive yeast K667 transformed with AcoCAX2 absorbed more calcium ions from the medium compared to the control. The promoter proNtPRB1b-proAcoCAX2 is specifically expressed in the junction between the stalk and fruit. Plants overexpressing AcoCAX2 in this junction absorbed more calcium in the fruit, resulting in a lower incidence of translucency compared to the control. Fruit with a mutant AcoCAX2 gene contained less calcium and exhibited a higher incidence of translucency than control. Conclusion The mutant flesh with AcoCAX2 contained more liquid in the intercellular space compared to the wild type and control. AcoCAX2 is the main gene responsible for transferring calcium ions from the stalk phloem into the fruit.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台