Immune Responses of Mango Callus Infected by Agrobacterium tumefaciens Inhibited Transformation
文献类型： 外文期刊
作者： Shu, Haiyan 1 ; Jewel, Zilhas Ahmed 6 ; Faruk, Omor 7 ; He, Luqiong 8 ; Wei, Qing 1 ; Zhan, Rulin 1 ; Chang, Shenghe 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 572025, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Peoples R China
3.Natl Key Lab Trop Crop Breeding, Sanya 572024, Peoples R China
4.Minist Agr & Rual Affairs, Key Lab Crop Gene Resources & Germplasm Enhancemen, Haikou 571101, Peoples R China
5.Key Lab Trop Crops Germplasm Resources Genet Impro, Haikou 571101, Peoples R China
6.Gopalganj Sci & Technol Univ, Fac Agr, Gopalganj 8100, Bangladesh
7.Gopalganj Sci & Technol Univ, Dept Biotechnol & Genet Engn, Gopalganj 8100, Bangladesh
8.Yunan Agr Univ, Coll Trop Crops, Puer 665099, Peoples R China
关键词： browning; gallic acid; infection efficiency; reactive oxygen species (ROS); type III secretion system gene cluster (T3SS)
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 11 期
页码：
收录情况： SCI
摘要： Mango is a vital fruit crop in tropical and subtropical regions, yet pests and diseases cause 30-70% production losses. Developing disease-resistant cultivars through transgenic methods could mitigate these issues. Agrobacterium-mediated callus transformation is a common genetic engineering approach, but successful transgenic mango plants from callus remain unreported due to severe browning and necrosis post-infection. We hypothesized that Agrobacterium-induced immune responses trigger callus death, hindering transformation. To improve efficiency, we engineered an Agrobacterium strain carrying the type III secretion system (T3SS) and effector gene AvrPto. Compared to controls, infected calluses exhibited elevated reactive oxygen species (ROS), along with up-regulated ROS-related, gallic acid biosynthesis, and defense genes. Calluses infected with T3SS-AvrPto-harboring Agrobacterium showed delayed browning and necrosis versus those infected with the empty vector (NV). The transformation rate with Agrobacterium (T3SS-AvrPto-EGFP) reached 1.6%, while Agrobacterium (NV-EGFP) failed entirely. These findings demonstrate that T3SS and AvrPto enhance mango transformation efficiency, offering a promising strategy for breeding multi-resistant varieties.
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