文献类型： 外文期刊
作者： Xu, Zhihao 1 ; Liu, Man 3 ; He, Qiyi 1 ; Li, Wenkai 1 ; Pan, Junkang 1 ; El-Sheikh, El-Sayed A. 4 ; Liu, Wenzhi 3 ; Hammock, Bruce D. 5 ; Li, Dongyang 1 ;
作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
2.Zhejiang Key Lab Intelligent Sensing & Robot Agr, Hangzhou 310058, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
4.Zagazig Univ, Fac Agr, Zagazig 44511, Egypt
5.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
6.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： Aquaculture; Nanobody; ELISA; GSIV; polyHRP; Immunoassay
期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.0； 五年影响因子：5.7 ）
ISSN： 0003-2670
年卷期： 2025 年 1350 卷
页码：
收录情况： SCI
摘要： Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus Ranavirus, is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control. In this context, a reliable diagnostic tool for the rapid detection of GSIV is crucial to mitigate its impact. This study developed a nanobody-based immunoassay for the rapid and reliable detection of GSIV. GSIV was cultured and used to immunize an alpaca, A phage library with an original diversity of 1.89 x 108 PFU was established, and six nanobodies were identified after three rounds of panning. Among them, HC-2 exhibited superior performance and was used to develop a highly sensitive ELISA method employing streptavidin-PolyHRP (SA-PolyHRP) as a signal amplification strategy. The assay achieved a detection limit of 3.3 x 105 PFU/mL and demonstrated high specificity without cross-reactivity. Practical application was validated in infected giant salamander samples, underscoring its diagnostic potential. This work provides a robust tool for GSIV diagnosis and showcases the potential of nanobodies in advancing aquaculture diagnostics.
- 相关文献
作者其他论文 更多>>
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Viperin and Its Effect on SVCV Replication in Common Carp, Cyprinus carpio
作者：Meng, Yan;Hu, Xi;Jiang, Nan;Fan, Yuding;Li, Yiqun;Xue, Mingyang;Xu, Chen;Liu, Wenzhi;Zhou, Yong
关键词：common carp (
Cyprinus carpio );viperin ; characteristics; antiviral activities -
Oyster-derived peptides as pancreatic lipase inhibitors: Kinetics, molecular interactions, and gastrointestinal stability
作者：Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Zhao, Yongqiang;Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Xu, Zhe
关键词：Oyster peptide; pancreatic lipase; inhibition mechanism; Molecular docking
-
Exploring Disparities in Gill Physiological Responses to NaHCO3-Induced Habitat Stress in Triploid and Diploid Crucian Carp (Carassius auratus): A Comprehensive Investigation Through Multi-Omics and Biochemical Analyses
作者：Han, Shicheng;Han, Lin;Yuan, Fangying;Liu, Wenzhi;Jin, Xiaofeng;Sun, Yanchun;Han, Lin;Liu, Wenzhi;Yuan, Fangying;Wang, Jing;Jin, Xiaofeng
关键词：crucian carp (
Carassius auratus ); diploid; triploid; carbonate alkalinity; metabolomics; transcriptomics -
Multi-omics-based study on metabolic-physiological response to heat stress in rainbow trout (Oncorhynchus mykiss)
作者：Yang, Yang;Wu, Song;Sun, Yanchun;Han, Shicheng;Liu, Yingjie;Liu, Wenzhi;Han, Lin;Yuan, Fangying;Wang, Jing
关键词：Rainbow trout; Heat stress; Multi-omics; Adaptive strategies; Inflammation response
-
Immunological Responses and Protection in the Largemouth Bass (Microterus salmoides) Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV)
作者：Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Zhou, Yong;Yang, Tao;Li, Chenyang;Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Li, Yiqun;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Huang, Zhenyu;Zhou, Yong;Jiang, Nan
关键词：largemouth bass; largemouth bass ranavirus; inactivated vaccine; relative percent survival rates; immune response
-
α-Ketoglutarate modulates the mechanisms of toxicity in crucian carp kidneys chronically exposed to NaHCO3: Metabolomics insights
作者：Liu, Wenzhi;Han, Lin;Liu, Qianwen;Sun, Yanchun;Liu, Wenzhi;Han, Lin;Yuan, Fangying;Liu, Qianwen;Cheng, Hongyu;Jin, Xiaofeng;Sun, Yanchun;Yuan, Fangying;Jin, Xiaofeng;Cheng, Hongyu
关键词：Crucian carp ( Carassius auratus ); alpha-Ketoglutarate; NaHCO 3; Metabolomics
