文献类型: 外文期刊
作者: Zhang, Weixiao 1 ; Deng, Suining 2 ; Zhao, Yan 1 ; Xu, Wei 1 ; Liu, Qingcai 2 ; Zhang, Yongzhong 1 ; Ren, Chunmei 3 ; Che 1 ;
作者机构: 1.Shandong Agr Univ, State Key Lab Crop Biol, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
2.China Agr Univ, Natl Maize Improvement Ctr, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
关键词: Maize; MRDD; QTL; Fine-mapping; MAS
期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.215; 五年影响因子:4.96 )
ISSN: 1471-2229
年卷期: 2021 年 21 卷 1 期
页码:
收录情况: SCI
摘要: BackgroundMaize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality. Resistance against MRDD is a complex trait that controlled by many quantitative trait loci (QTL) and easily influenced by environmental conditions. So far, many studies have reported numbers of resistant QTL, however, only one QTL have been cloned, so it is especially important to map and clone more genes that confer resistance to MRDD.ResultsIn the study, a major quantitative trait locus (QTL) qMrdd2, which confers resistance to MRDD, was identified and fine mapped. qMrdd2, located on chromosome 2, was consistently identified in a 15-Mb interval between the simple sequence repeat (SSR) markers D184 and D1600 by using a recombinant inbred line (RIL) population derived from a cross between resistant ("80007") and susceptible ("80044") inbred lines. Using a recombinant-derived progeny test strategy, qMrdd2 was delineated to an interval of 577 kb flanked by markers N31 and N42. We further demonstrated that qMrdd2 is an incompletely dominant resistance locus for MRDD that reduced the disease severity index by 20.4%.ConclusionsA major resistance QTL (qMrdd2) have been identified and successfully refined into 577 kb region. This locus will be valuable for improving maize variety resistance to MRDD via marker-assisted selection (MAS).
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