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Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation in Hypsizygus marmoreus

文献类型： 外文期刊

作者： Gong, Ming 1 ; Huang, Tianyu 3 ; Li, Yan 1 ; Li, Jinxin 4 ; Tang, Lihua 1 ; Su, Erzheng 2 ; Zou, Gen 1 ; Bao, Dapeng 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi,Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Shanghai 201403, Peoples R China

2.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Dept Food Sci & Technol, Nanjing 210037, Peoples R China

3.Jiangsu Ocean Univ, Coll Food Sci & Engn, Lianyungang 222005, Peoples R China

4.Shanghai Finc Biotech Inc, Res & Dev Ctr, Shanghai 201401, Peoples R China

关键词： low-temperature fruiting; multi-omics; cultivation; GCN2; Hypsizygus marmoreus

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 7 期

页码：

收录情况： SCI

摘要： Hypsizygus marmoreus is a representative edible mushroom with low-temperature fruiting after a long postripening (LFLP). Clarifying the mechanism of LFLP and applying a rigorous low-temperature-limited process will optimize the mushroom cultivation process. This study performed an integrative multi-omics analysis of the molecular mechanism of LFLP in combination with genetic, physiological, and cultivation confirmation. The results showed that the amino acid content was increased during LFLP, mainly arginine. pH analysis showed acidification in the postripening stage and alkalization in the substrates of the reproductive growth stage. An enzyme activity test confirmed the increased enzyme activity of arginase and citrate synthase in the postripening stage. Weighted gene coexpression network analysis of the transcriptome and metabolomics indicated that pH variation is correlated mainly with changes in citrate and arginine. Multi-omics reveals a straightforward way of providing enriched materials for amino acid biosynthesis, namely, synergistically elevating citric acid and arginine through enhanced activity of the arginine synthesis branch pathway in the citrate cycle. Our study confirmed that GCN2 mediated metabolic adaptation by enhancing protein translation, highlighting its regulatory role during LFLP. Exogenously added citric acid and arginine shortened the postripening period by 10 days and increased the fruiting body yield by 10.2 similar to 15.5%. This research sheds light on the molecular mechanism of LFLP in H. marmoreus and highlights the promising application of nutrient accumulation in high-efficiency cultivation.

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