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Chlorogenic acid accumulation and related gene expression in peach fruit

文献类型： 外文期刊

作者： Yan, Juan 1 ; Su, Ziwen 1 ; Guo, Shaolei 1 ; Zhang, Minghao 3 ; Zhang, Binbin 1 ; Cai, Zhixiang 1 ; Shen, Zhijun 1 ; Ma, Ruijuan 1 ; Yu, Mingliang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Pomol, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

3.Shanghai Acad Agr Sci, Forest & Fruit Tree Res Inst, Shanghai 201403, Peoples R China

关键词： Prunus persica (L; ); Chlorogenic acids; RNA-seq; Gene expression; HCT

期刊名称：HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY （ 影响因子：2.138； 五年影响因子：2.34 ）

ISSN： 2211-3452

年卷期： 2022 年 63 卷 3 期

页码：

收录情况： SCI

摘要： To reveal the molecular mechanism in the accumulation of chlorogenic acids (CGAs) in peach (Prunus persica) fruit during growth and development, CGA contents in the flesh of the three peach cultivars 'Ruiguang 18', 'Heiyoutao', and 'Beijingyixianhong' were determined. The expression levels of CGA metabolism-related genes were analyzed based on transcriptome data (RNA-seq). These candidate genes were then screened and real-time fluorescent quantitative PCR (qRT-PCR) was performed to verify their expression. The results showed that the content of total CGAs, 5-O-caffeoylquinic acid and 3-O-caffeoylquinic acid, in the flesh of 'Ruiguang 18' exhibited a decreasing trend during fruit development, and there was a great drop at maturity stage (P < 0.05). The three contents in 'Heiyoutao' increased first at stage S2 (P < 0.05) and then decreased significantly (P < 0.05). In 'Beijingyixianhong', they stayed stable in the early stages, then total CGAs and 3-O-caffeoylquinic acid decreased significantly at the maturity stage (P < 0.05). RNA-seq transcriptome data analysis and qRT-PCR expression analysis showed that the accumulation of CGAs in fruit flesh was mainly affected by the expression of Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL), and their expression levels were highly consistent with total CGA content. Thus, we concluded that Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL) are the key genes for CGAs synthesis in peach flesh.

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