Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil
文献类型： 外文期刊
作者： Fang, Xuechen 1 ; Liu, Tingting 1 ; Xue, Chang 3 ; Xue, Guangjian 1 ; Wu, Mengyun 1 ; Liu, Pengyan 2 ; Hammock, Bruce D. 4 ; Lai, Weihua 1 ; Peng, Juan 1 ; Zhang, Cunzheng 2 ;
作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China
2.Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety,State Key Lab C, Nanjing 210014, Peoples R China
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
4.Univ Calif Davis, Dept Entomol, Nematol, Davis, CA 95616 USA
5.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： Fluorescent magnetic nanobeads; Aggregation-induced emission; Immunomagnetic separation; Lateral flow immunoassay; Ratiometric fluorescence
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 433 卷
页码：
收录情况： SCI
摘要： In this study, we develop a competitive ratiometric fluorescent lateral flow immunoassay (CRF-LFIA) based on dual emission fluorescence signal, which has great advantage in visual and quantitative detection of Chlorothalonil (CTN). Red-emitted fluorescent magnetic nanobeads (FMNBs) and green-emitted aggregation-induced emission fluorescent microsphere (AIEFM) are synthesized and conjugated to antibodies and antigens respectively, resulting in competitive binding with the analyte. The ratiometric fluorescence signal which comes from the overlap of these two fluorescence emissions. FMNBs probes also provide immunomagnetic separation (IMS) to enrich the analysts and resist complex matrix effects. This strip generates a visually discernible yellow-to-green fluorescence color change in the presence of CTN (2 ng/mL), which could be incisively observed by naked eye. Moreover, the limit of detection (LOD) reached 0.152 ng/mL by measurement of color (Red-Green-Blue, RGB) signals. Method validation shows a good correlation between CRF-LFIA and LC-MS/MS.
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