Alteration of antioxidant status, glucose metabolism, and hypoxia signal pathway in Eirocheir sinensis after acute hypoxic stress and reoxygenation
文献类型： 外文期刊
作者： Chen, Xue 1 ; Feng, Wenrong 2 ; Yan, Fengyuan 1 ; Li, Wenjing 3 ; Xu, Pao 2 ; Tang, Yongkai 1 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China
3.Jiangsu Haorun Biol Ind Grp Co Ltd, Taizhou 225300, Peoples R China
4.Jiangsu Haorun Natl Crab Seed Technol Co Ltd, Taizhou 225300, Peoples R China
关键词： Hypoxic stress; Eirocheir sinensis; Antioxidative response; Glycolysis; Hypoxia signaling pathway
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.9； 五年影响因子：3.8 ）
ISSN： 1532-0456
年卷期： 2023 年 268 卷
页码：
收录情况： SCI
摘要： Dissolved oxygen (DO) is crucial for the survival of Chinese mitten crab (Eirocheir sinensis); low DO levels adversely affect the health of these crabs. In this study, we evaluated the underlying response mechanism of E. sinensis to acute hypoxic stress by analyzing antioxidant parameters, glycolytic indicators, and hypoxia signaling factors. The crabs were exposed to hypoxia for 0, 3, 6, 12, and 24 h and reoxygenated for 1, 3, 6, 12, and 24 h. The hepatopancreas, muscle, gill, and hemolymph were sampled at different exposure times to detect the biochemical parameters and gene expression. The results showed that the activity of catalase, antioxidants, and malondialdehyde in tissues significantly increased under acute hypoxia and gradually decreased during the reoxygenation phase. Under acute hypoxic stress, glycolysis indices, including hexokinase (HK), phosphofruc-tokinase, pyruvate kinase (PK), pyruvic acid (PA), lactate dehydrogenase (LDH), lactic acid (LA), succinate dehydrogenase (SDH), glucose, and glycogen in the hepatopancreas, hemolymph, and gills increased to varying degrees but recovered to the control levels after reoxygenation. Gene expression data showed that hypoxia signaling pathway-related genes, including hypoxia-inducible factor-1 & alpha;/& beta; (HIF1 & alpha;/& beta;), prolyl hydroxylase (PHD), factor inhibiting hypoxia-inducible factor (FIH), and glycolysis-related factors (HK and PK) were upregulated, showing that the HIF signaling pathway was activated under hypoxic conditions. In conclusion, acute hypoxic exposure activated the antioxidant defense system, glycolysis, and HIF pathway to respond to adverse conditions. These data contribute to elucidating the defense and adaptive mechanisms of crustaceans to acute hypoxic stress and reoxygenation.
- 相关文献
作者其他论文 更多>>
-
Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis
作者：Jiang, Gang;Yan, Fengyuan;Xu, Yuanfeng;Li, Jianlin;Feng, Wenrong;Tang, Yongkai;Tang, Yongkai;Hua, Guo an;Li, Wen jing;Zhou, Jun
关键词：Chinese mitten crab; Hypoxic stress; Aeromonas veronii infection; Immunity; Metabolism
-
Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via Bursicon in the Cerebral Ganglion of Chinese Mitten Crab Eriocheir sinensis Under Alkaline Stress
作者：Wang, Meiyao;Xu, Gangchun;Tang, Yongkai;Wang, Meiyao;Xu, Gangchun;Tang, Yongkai;Zhou, Jun;Ge, Jiachun
关键词：
Eriocheir sinensis ; cerebral ganglia; alkalinity stress;bursicon ; signal transduction -
Comparative transcriptome analysis of gill tissue response to hypoxia stress in the Chinese mitten crab ( Eriocheir sinensis)
作者：Chen, Xue;Bai, Yulin;Wang, Anqi;Xu, Dongpo;Ma, Junlei;Xu, Dongpo
关键词：Transcriptome; Eriocheir sinensis; Hypoxia; Oxidative stress; Apoptosis
-
Transcriptomic profiling reveals potential regulatory genes and molecular mechanisms of residual feed intake in Jian carp (Cyprinus carpio var. Jian)
作者：Wang, Wenqian;Jiang, Kemeng;Li, Jianlin;Feng, Wenrong;Sewo, Daniel Yohannes;Tang, Yongkai;Xu, Yuanfeng;Li, Jianlin;Feng, Wenrong;Tang, Yongkai;Zhang, Zhihua
关键词：Cyprinus carpio; Feed efficiency; Residual feed intake; Transcriptome
-
Variability in Morphological Traits and Nutritional Profiles of Adult Eriocheir sinensis in Different Aquacultural Regions
作者：Feng, Wenrong;He, Qinghong;Li, Jianlin;Xu, Yuanfeng;Jiang, Gang;Tang, Yongkai;Zhou, Jun;Hua, Guoan;Tang, Yongkai
关键词：
Eriocheir sinensis ; aquaculture; morphometrics; amino acid; fatty acid -
Effects of starvation-refeeding on antioxidant status, metabolic function, and adaptive response in the muscle of Cyprinus carpio
作者：Feng, Wenrong;Li, Jianlin;Xu, Yuanfeng;Jiang, Gang;Su, Shengyan;Jia, Rui;Tang, Yongkai;Zhang, Zhihua
关键词：Starvation; Oxidative stress; Mitophagy; FoxO signaling pathway; Cyprinus carpio
-
Physiological response and lipid metabolism induced by re-feeding in Jian carp (Cyprinus carpio var. Jian) subjected to short-term starvation
作者：Zhang, Yu;Tang, Yongkai;Jiang, Gang;Feng, Wenrong;Xu, Yuanfeng;Li, Jianlin;Tang, Yongkai;Zhang, Zhihua
关键词：Cyprinus carpio var. Jian; Starvation and refeeding; Growth performance; Physiological response; Lipid metabolism
