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Genomic Characteristics and Pathogenicity of Novel Reassortant Mammalian Orthoreoviruses From Sheep, China

文献类型： 外文期刊

作者： Li, Xia 1 ; Cai, Xuhang 1 ; He, Yi 7 ; Li, Wenliang 1 ; Zhai, Junjun 8 ; Luo, Runbo 5 ; Suolang, Sizhu 5 ; Mao, Li 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Vet Biol Engn & Technol, Nanjing, Peoples R China

3.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Nanjing, Peoples R China

4.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing, Peoples R China

5.Xizang Agr & Anim Husb Univ, Linzhi, Peoples R China

6.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

7.Peoples Govt Chengguan Town, Agr Dev Serv Ctr, Fukang, Peoples R China

8.Yulin Univ, Shaanxi Prov Engn & Technol Res Ctr Cashmere Goat, Yulin, Peoples R China

关键词： mammalian orthoreoviruses (MRVs); pathogenicity; reassortment; sheep; virus isolation and identification

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：3.0； 五年影响因子：3.4 ）

ISSN： 1865-1674

年卷期： 2025 年 2025 卷 1 期

页码：

收录情况： SCI

摘要： Mammalian orthoreoviruses (MRVs) have a wide geographic distribution worldwide and have been detected from humans and a variety of animal species. This study represents the first isolation of MRV from sheep rectal swabs in China, with analyses of its molecular and pathogenicity characteristics. MRV-positive samples were inoculated into Madin-Darby bovine kidney (MDBK) cells, resulting in stable cytopathic effects (CPEs) after three generations of blind passage. Two isolates were isolated and confirmed as MRV, named MRV-XJ23 and MRV-sheep/SY13, through reverse-transcription polymerase chain reaction (RT-PCR), transmission electron microscopy, and indirect immunofluorescence assay (IFA). The viruses exhibited broad cellular tropism. Whole-genome sequences were obtained and subjected to homology and evolutionary analyses, revealing that MRV-XJ23 and MRV-sheep/SY13 belong to the MRV-1 serotype. Phylogenetic analyses demonstrated that MRV-XJ23 is a reassortant virus containing gene segments from three MRVs that infected humans, bovines, and bats, with nucleotide homology exceeding 94.56%. The gene segments of MRV-sheep/SY13 were derived from five strains-Osaka2005, BatMRV-2/SNU1/Korea/2021, T1/human/Netherlands/1/84, IND/MZ/3013814/reo, and B/03-with nucleotide homology exceeding 95.47%. Animal experiments demonstrated that MRV-sheep/SY13 infection induced significant pathological changes in the respiratory and digestive tracts of mice. In sheep, MRV-sheep/SY13 caused respiratory infections, but no obvious lesion was observed from the digestive tract. This study expands our understanding of the MRV host range, reveals the potential public health risk of MRV transmission across species and zoonotic transmission, and underscores the necessity of further studies on epidemiology, reassortment patterns, and pathogenicity of MRV in sheep and domestic animals.

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