α-Glycerol Monolaurate Promotes Tight Junction Assembly and Enhances Epithelial Barrier Function in IPEC-J2 Cells and Partridge Chicks
文献类型： 外文期刊
作者： Dai, Siyu 1 ; Meng, Shuai 1 ; Liu, Shifeng 1 ; Li, Dagang 2 ; Wang, Hong 1 ;
作者机构： 1.Jinan Univ, Coll Life Sci & Technol, Guangdong Prov Engn Res Ctr Antibody Drug & Immuno, Guangzhou, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Guangdong, Peoples R China
关键词： CaSR/PLC/IP3/AMPK signaling pathway; intestinal epithelia; tight junction assembly; alpha-glycerol monolaurate
期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.2； 五年影响因子：1.9 ）
ISSN： 1344-3941
年卷期： 2025 年 96 卷 1 期
页码：
收录情况： SCI
摘要： This study investigated the role and mechanism of alpha-glycerol monolaurate (alpha-GML), a Food and Drug Administration-approved natural emulsifier, in enhancing tight junction (TJ) assembly and intestinal barrier function. In vitro experiments showed that alpha-GML significantly increased the gene and protein levels of TJ-related occludin (OCLN) and zonula occluden-1 (ZO-1), increased ZO-1 distribution in the cell membrane, and reduced paracellular permeability in porcine jejunum epithelial cells (IPEC-J2). These findings indicated that alpha-GML enhanced intestinal barrier function by promoting TJ protein expression and assembly. Compared with the alpha-GML group, TJ protein expression and assembly and AMP-activated protein kinase (AMPK) phosphorylation were significantly downregulated in cotreatment groups receiving alpha-GML along with a calcium-sensing receptor antagonist or a phospholipase C inhibitor. Notably, the groups treated with alpha-GML and inositol 1,4,5-trisphosphate receptor inhibitor showed a significant decrease in TJ assembly and AMPK phosphorylation, with no significant difference in the TJ protein expression levels. In vivo experiments demonstrated that dietary alpha-GML significantly upregulated TJ levels and AMPK phosphorylation in the jejunum of partridge chicks. In conclusion, alpha-GML may upregulate intestinal epithelial TJ assembly via AMPK activation and promote intestinal barrier development in young animals to maintain intestinal health.
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