Investigating the Potential Mechanism of Methane Mitigation in Seaweed Gracilaria lemaneiformis via 16S rRNA Gene Sequencing and LC/MS-Based Metabolomics
文献类型： 外文期刊
作者： Sun, Yi 1 ; Li, Shuai 1 ; Guo, Tongjun 3 ; Tong, Xiong 1 ; Zhang, Zhifei 1 ; Yang, Yufeng 2 ; Wang, Qing 2 ; Li, Dagang 1 ; Min, Li 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Minist Agr,Key Lab Anim Nutr & Feed Sci South Chin, Guangzhou 510640, Peoples R China
2.Jinan Univ, Dept Ecol, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Guangzhou 510632, Peoples R China
3.Xinjiang Acad Anim Sci, Feed Res Inst, Key Lab Xinjiang Feed Biotechnol, Urumqi 830000, Peoples R China
4.Agrifood & Biosci Inst, Hillsborough BT26 6DR, North Ireland
关键词： Gracilaria lemaneiformis; methane; rumen fermentation; microbial sequencing; metabolomics
期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 15 卷 16 期
页码：
收录情况： SCI
摘要： Methane (CH4), originating from ruminants, is a major source of greenhouse gas emissions in the agriculture industry. This study aimed to determine the potential of red seaweed Gracilaria lemaneiformis (G. lemaneiformis) as an anti-methanogenic feed additive for cattle. Three supplementation levels of seaweed (2%, 5%, and 10% of dry matter) were evaluated for their effects on gas production and rumen fermentation characteristics during 48 h in vitro fermentation. The results revealed a significant decrease in total gas production (TGP), CO2, CH4, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, with no differences in pH or dry matter disappearance (DMD). Notably, compared with the control group without seaweed, supplementation with 2% G. lemaneiformis effectively reduces CH4 emissions by 27.5% (p < 0.05). Supplementation with 2% G. lemaneiformis decreased the abundance of methanogens g_norank_f_Methanomethylophilaceae, responsible for CH4 generation, and increased the populations of bacteria (Kandleria and Succinivibrio) that compete with methanogens for substrates. Furthermore, upregulating the levels of 13(S)-HOTrE and 9(S)-HOTrE (polyunsaturated fatty acids) could inhibit methanogenic activity. Additionally, lower VFA concentrations will provide less raw materials for methane synthesis, thus further inhibiting methanogenesis. In summary, G. lemaneiformis, as a red seaweed with important economic value, can not only be applied to enhance marine carbon sinks but can also serve as a promising candidate for mitigating biomethane emissions in cattle.
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